Galactosylated chitosan-graft-poly(ethylene glycol) as hepatocyte-targeting DNA carrier
Galactosylated chitosan-graft-poly(ethylene glycol) as hepatocyte-targeting DNA carrier
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DOI：
10.1016/s0168-3659(01)00448-5
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发表时间：
2001-10-19
影响因子：
10.8
通讯作者：
Cho, CS
中科院分区：
文献类型：
--
作者：
Park, IK;Kim, TH;Cho, CS
将带有半乳糖基的乳糖酸与壳聚糖偶联以获得肝特异性，并将聚乙二醇（PEG）接枝到半乳糖基化壳聚糖（GC）上以提高其在水中的稳定性和细胞通透性。通过琼脂糖凝胶电泳证实了半乳糖基化壳聚糖-接枝-PEG（GCP）/DNA复合物的形成。与GC/DNA复合物相比，GCP/DNA复合物的稳定性有所提高。GCP/DNA复合物的粒径随着GCP与DNA的电荷比的增加而减小，在电荷比为5时，复合物的粒径在27 nm左右达到最小值。与DNA本身的构象相比，与GCP形成复合物后，DNA的构象没有发生变化。GCP/DNA复合物仅转染到具有脱唾液酸糖蛋白受体（ASGR）的Hep G2中，这表明细胞上的ASGR和GCP上的半乳糖配体的特异性相互作用。(C)2001 Elsevier Science B. V.保留所有权利。
Lactobionic acid bearing galactose group was coupled with chitosan for liver specificity, and poly(ethylene glycol) (PEG) was grafted to galactosylated chitosan (GC) for stability in water and enhanced cell permeability. Complex formation of galactosylated chitosan-graft-PEG (GCP)/DNA complexes was confirmed by agarose gel electrophoresis. Compared to GC/DNA complex, the stability of GCP/DNA complex could be enhanced. Particle sizes of GCP/DNA complexes decreased as the charge ratio of GCP to DNA increased and had a minimum value around 27 nm at the charge ratio of 5. Conformational change of DNA did not occur after complex formation with GCP compared to conformation of DNA itself. GCP/DNA complexes were only transfected into Hep G2 having asialoglycoprotein receptors (ASGR), indicative of specific interaction of ASGR on cells and galactose ligands on GCP. (C) 2001 Elsevier Science B.V. All rights reserved.