The effects of lateral hypothalamic-medial forebrain stimulation and somatostatin antiserum on pulsatile growth hormone secretion in freely behaving rats: evidence for a dual regulatory mechanism.
The effects of lateral hypothalamic-medial forebrain stimulation and somatostatin antiserum on pulsatile growth hormone secretion in freely behaving rats: evidence for a dual regulatory mechanism.
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下丘脑外侧-内侧前脑刺激和生长抑素抗血清对自由行为大鼠脉动生长激素分泌的影响：双重调节机制的证据。
DOI：
10.1210/endo-109-2-622
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发表时间：
1981
期刊：
影响因子：
4.8
通讯作者：
J. B. Martin
中科院分区：
文献类型：
--
作者：
L. C. Terry;J. B. Martin
本研究的目的是研究生长抑素（SRIF）和电刺激外侧下丘脑-内侧前脑束（LH-MFB）对自由行为，长期插管植入脑电极的雄性大鼠脉冲式GH分泌动力学的作用。还研究了抗SRIF血清（AS-SRIF）给药对脉冲GH以及TSH和PRL分泌的影响。其结果是：1）循环AS-SRIF增加自由行为大鼠的GH谷水平，但对GH分泌爆发的幅度或平均GH水平没有显著影响; 2）如果在循环GH水平低时给予LH-MFB刺激，则可以刺激GH释放; 3）如果在自发GH爆发时给予LH-MFB刺激，则抑制GH分泌; 4）AS-SRIF预处理可阻止刺激诱导的GH抑制，表明该反应由内源性SRIF介导; 5）AS-SRIF可增加TSH分泌，但对PRL无影响。这些结果提供了证据，以支持这一假设，垂体GH分泌的兴奋性和抑制性的影响，其中的抑制成分是由SRIF介导的组合调节。
The purpose of this investigation was to examine the role of somatostatin (SRIF) and electrical stimulation of the lateral hypothalamic-medial forebrain bundle (LH-MFB) on dynamics of pulsatile GH secretion in freely behaving, chronically cannulated male rats with implanted brain electrodes. The effects of administration of anti-SRIF serum (AS-SRIF) on pulsatile GH and on TSH and PRL secretion was also studied. The results are: 1) circulating AS-SRIF increases trough levels of GH in freely behaving rats but has no significant effect on the amplitude of GH secretory bursts or mean GH levels; 2) LH-MFB excitation can stimulate GH release if delivered when circulating GH levels are low; 3) LH-MFB stimulation inhibits secretion of GH if given at the time of a spontaneous GH burst; 4) stimulation-induced GH inhibition is prevented by pretreatment with AS-SRIF, suggesting that this response is mediated by endogenous SRIF; and 5) AS-SRIF increases TSH secretion but has no effect on PRL. These results provide evidence to support the hypothesis that pituitary GH secretion is regulated by a combination of excitatory and inhibitory influences, the inhibitory component of which is mediated by SRIF.