Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method
Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method
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DOI：
10.1007/978-1-4939-1363-3_1
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发表时间：
2014-01-01
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影响因子：
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通讯作者：
Gietz, R. Daniel
中科院分区：
文献类型：
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作者：
Gietz, R. Daniel
酵母的高效转化是酵母分子生物学研究的基础。它对于酵母双杂交系统和基因组修饰等技术至关重要。在这里，我将介绍用于许多不同转换应用程序的协议。一个快速和简单的协议是在不需要大量的转化体的情况下。高效方案从少量DNA产生大量转化体，其可以针对文库转化方案按比例放大。还包括微量滴定板格式的转化方案。最后，包括用于生产冷冻感受态酵母细胞的方案，该方案允许细胞从冷冻机中取出并以高效率转化。
Efficient transformation of yeast has been the corner stone of molecular studies in yeast for numerous years. It is essential for techniques such as the yeast two-hybrid system as well as genome modification. Here I present protocols for a number of different transformation applications. A quick and easy protocol is for situations in which large numbers of transformants are not required. The high-efficiency protocol generates large numbers of transformants from small amounts of DNA that can be scaled up for the library transformation protocol. A transformation protocol for a microtiter plate format is also included. Finally, a protocol for the production of frozen competent yeast cells is included, which allows cells to be taken from the freezer at a moment's notice and transformed with high efficiency.