Proanthocyanidin-rich grape seed extract improves bone loss, bone healing, and implant osseointegration in ovariectomized animals
Proanthocyanidin-rich grape seed extract improves bone loss, bone healing, and implant osseointegration in ovariectomized animals
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DOI：
10.1038/s41598-020-65403-4
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发表时间：
2020-06-01
影响因子：
4.6
通讯作者：
Nakamura, Keisuke
中科院分区：
文献类型：
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作者：
Tenkumo, Taichi;Aobulikasimu, Alkebaier;Nakamura, Keisuke
本研究的目的是证实富含原花青素的葡萄籽提取物(GSE)是否能够改善去卵巢(OVX)动物的骨丢失、骨愈合和种植体骨结合(定义为骨组织与种植体之间的直接连接)等骨健康状况。我们证明，每天口服GSE可以防止去卵巢小鼠腰椎和股骨的骨丢失。此外，通过组织学和组织形态计量学分析，OVX小鼠腰椎骨中的破骨细胞生成被加速，但GSE阻止了这种动态，这表明GSE可以抵消OVX诱导的加速破骨细胞生成活动。在大鼠中，OVX明显损害了颅骨上产生的缺陷的愈合，而GSE克服了OVX损害的愈合。同样，大鼠的胫骨植入物的骨结合被OVX抑制，而GSE抵消了OVX诱导的骨结合不良，可能通过防止不平衡的骨转换来促进骨愈合。这些结果表明，口服GSE通过减轻OVX作为雌激素缺乏模型引起的骨健康损害来改善种植体的骨整合。
The purpose of the present study was to confirm if proanthocyanidin-rich grape seed extract (GSE) had the ability to improve bone health such as bone loss, bone healing, and implant osseointegration (defined as the direct connection between bone tissue and an implant) in ovariectomized (OVX) animals. We demonstrated that daily oral administration of GSE prevented bone loss in the lumbar vertebrae and femur in OVX mice. In addition, osteoclastogenesis in the lumbar spine bone of OVX mice, as assessed by histological and histomorphometric analyses, was accelerated but GSE prevented this dynamization, suggesting that GSE could counteract OVX-induced accelerated osteoclastogenic activity. In rats, OVX clearly impaired the healing of defects created on the calvaria, and GSE overcame this OVX-impaired healing. In the same way, osseointegration of a tibial implant in rats was retarded by OVX, and GSE counteracted the OVX-induced poor osseointegration, likely promoting bone healing by preventing imbalanced bone turnover. These results suggest that orally administered GSE improved implant osseointegration by mitigating the impaired bone health induced by OVX as a model of estrogen deficiency.