cpbAg1 encodes an active carboxypeptidase B expressed in the midgut of Anopheles gambiae
cpbAg1 encodes an active carboxypeptidase B expressed in the midgut of Anopheles gambiae
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DOI：
10.1111/j.1365-2583.2004.00541.x
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发表时间：
2005-04-01
影响因子：
2.6
通讯作者：
Bourgouin, C
中科院分区：
文献类型：
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作者：
Lavazec, C;Bonnet, S;Bourgouin, C
我们以前使用的差异显示，以确定几个冈比亚按蚊基因，其在蚊子中肠的表达调节后，恶性疟原虫的摄入。在这里，我们报告的这些基因之一，cpbAg 1，它的第一个锌羧肽酶B的代码中确定的一个特征。冈比亚和任何昆虫。cpbAg 1在杆状病毒中的表达产生了一种活性酶，N-末端氨基酸的测定证实，CPBAg 1含有一个信号肽和一个前肽，这是消化锌羧肽酶的典型特征。cpbAg 1 mRNA主要产生于蚊子中肠，在那里它积累在未进食的雌性中，并在吸血后迅速下调。安的注解。gambiae基因组预测了编码锌羧肽酶的23个序列，其中只有两个（cpbAg 1和cpbAg 2）在蚊子中肠中以显著水平表达。
We previously used differential display to identify several Anopheles gambiae genes, whose expression in the mosquito midgut was regulated upon ingestion of Plasmodium falciparum. Here, we report the characterization of one of these genes, cpbAg1, which codes for the first zinc-carboxypeptidase B identified in An. gambiae and in any insect. Expression of cpbAg1 in baculovirus gave rise to an active enzyme, and determination of the N-terminal amino acids confirmed that CPBAg1 contains a signal peptide and a pro-peptide, typical features of digestive zinc carboxypeptidases. cpbAg1 mRNA was mainly produced in the mosquito midgut, where it accumulated in unfed females and was rapidly down-regulated upon blood feeding. Annotation of the An. gambiae genome predicts twenty-three sequences coding for zinc-carboxypeptidases of which only two (cpbAg1 and cpbAg2) are expressed at a significant level in the mosquito midgut.