A new approach to study exhaled proteins as potential biomarkers for asthma
A new approach to study exhaled proteins as potential biomarkers for asthma
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DOI：
10.1111/j.1365-2222.2010.03638.x
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发表时间：
2011-03-01
影响因子：
6.1
通讯作者：
Schoeters, G.
中科院分区：
文献类型：
--
作者：
Bloemen, K.;Van Den Heuvel, R.;Schoeters, G.
背景哮喘是一种以气道炎症为主要特征的复杂临床疾病.最近，各种研究报道了呼出气冷凝物（EBC）的分析，以寻找哮喘的潜在生物标志物。然而，在复杂的疾病如哮喘中，一种生物标志物可能不足以进行早期诊断或随访。蛋白质组分析的使用可以揭示疾病特异性蛋白水解肽或蛋白质模式，方法采用高效液相色谱-质谱联用技术分离和检测哮喘相关蛋白质（蛋白水解肽）存在于EBC样品从30名健康儿童和40名儿童哮喘在6-12 years.ResultsSupport向量机分析导致分化配置文件哮喘状态的基础上。这些蛋白水解肽模式与一些众所周知的（肺活量测定法，呼出一氧化氮）和最近描述的呼出标志物（EBC pH，LTB 4）不相关。EBC中更丰富的蛋白质被确定为细胞角蛋白，白蛋白，肌动蛋白，血红蛋白，溶菌酶，杀皮素，和钙颗粒蛋白B。ConclusionAlthough的确切作用，在疾病的发展或所提出的模式中描述的蛋白质的气道的生理状态目前还不清楚，这是一个重要的步骤，在寻找呼出的生物标志物哮喘。这项研究表明，EBC含有对未来非侵入性哮喘诊断或随访感兴趣的蛋白质。
P>BackgroundAsthma is a complex clinical disease characterized by airway inflammation. Recently, various studies reported on the analysis of exhaled breath condensate (EBC) in the search for potential biomarkers for asthma. However, in a complex disease such as asthma, one biomarker might not be enough for early diagnosis or follow-up.ObjectiveThe use of proteome analysis may reveal disease-specific proteolytic peptide or protein patterns, and may lead to the identification of novel proteins for the detection of asthma.MethodsLiquid chromatography and mass spectrometry were used to separate and detect proteins (proteolytic peptides) present in EBC samples from 30 healthy children and 40 children with asthma in the age group of 6-12 years.ResultsSupport vector machine analysis resulted in differentiating profiles based on asthma status. These proteolytic peptide patterns were not correlated to some well known (spirometry, exhaled nitric oxide) and more recently described exhaled markers (EBC pH, LTB4). The more abundant proteins in EBC were identified as cytokeratins, albumin, actin, haemoglobin, lysozyme, dermcidin, and calgranulin B.ConclusionAlthough the exact role in the disease development or physiological state of the airways of the proteins described in the presented pattern is not clear at this moment, this is an important step in the search for exhaled biomarkers for asthma. This study shows that EBC contains proteins that are of interest for future non-invasive asthma diagnosis or follow-up.