Europium-labeled activity-based probe through click chemistry: absolute serine protease quantification using (153)Eu isotope dilution ICP/MS.
Europium-labeled activity-based probe through click chemistry: absolute serine protease quantification using (153)Eu isotope dilution ICP/MS.
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DOI：
10.1002/anie.201108277
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发表时间：
2012-04
影响因子：
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通讯作者：
Xiaowen Yan;Yacui Luo;Zhubao Zhang;Zhaoxin Li;Q. Luo;Limin Yang;Bo Zhang;Haifeng Chen;
中科院分区：
文献类型：
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作者：
Xiaowen Yan;Yacui Luo;Zhubao Zhang;Zhaoxin Li;Q. Luo;Limin Yang;Bo Zhang;Haifeng Chen;
点击并分析：利用点击化学法将磺酰氟与叠氮单元偶联，合成了能与丝氨酸蛋白酶（SP）活性部位的丝氨酸（Ser）特异性结合的SF-Eu探针。该方法与（153）Eu同位素稀释ICP/MS的组合使得仅使用一种（153）Eu（NO（3））（3）同位素标准物就能够对生物样品中的活性SP进行绝对蛋白质定量。
Click and analyze: the titled probe was synthesized by conjugating a sulfonyl fluoride and azido unit using click chemistry to give SF-Eu, which can react specifically with serine (Ser) in the active site of serine protease (SP). Combination of the method with (153)Eu-isotope dilution ICP/MS enables absolute protein quantification of active SPs in biological samples using only one (153)Eu(NO(3))(3) isotopic standard.