A Novel Proposal of Salivary Lymphocyte Detection and Phenotyping in Patients Affected by Sjogren's Syndrome
A Novel Proposal of Salivary Lymphocyte Detection and Phenotyping in Patients Affected by Sjogren's Syndrome
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DOI：
10.3390/jcm9020521
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发表时间：
2020-02-01
影响因子：
3.9
通讯作者：
Turkina, Anna
中科院分区：
文献类型：
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作者：
Selifanova, Elena;Beketova, Tatjana;Turkina, Anna
对干燥综合征(SS)患者腮腺分泌物细胞成分进行了初步评价，并对唾液淋巴细胞检测和免疫表型在干燥综合征诊断和预后中的诊断准确性进行了初步评价。这项研究包括40名年龄19-60岁的干燥综合征相关的实质性涎腺炎患者和20名健康献血者。排除标准为涎腺炎加重、慢性感染、恶性肿瘤和淋巴增生性疾病。标准的诊断试验是小唾液腺活检和腮腺造影。用多色流式细胞仪对腮腺分泌物淋巴细胞进行免疫表型分析。干燥综合征患者腮腺分泌物中可检测到原发(PSS)和继发性(SSS)淋巴细胞，而正常人腮腺分泌物中未检测到淋巴细胞。腮腺唾液淋巴细胞检测的敏感性、特异性、阳性预测值和阴性预测值分别为77.5%、100%、100%和69%。T细胞总数、辅助性T细胞和T细胞毒性细胞的平均数分别为71.7%、41.6%和53%。从患者腮腺血流中获得的淋巴细胞的免疫表型与目前已知的腺体活检的免疫表型相似。我们的初步数据表明，唾液可作为评估干燥综合征预后的一种非侵入性替代方法。
A preliminary evaluation of the parotid secretion cellular composition in patients with Sjogren's Syndrome (SS) and a diagnostic accuracy assessment of salivary lymphocyte detection and immunophenotyping in Sjogren's Syndrome diagnosis and prognosis were performed. The study included 40 consecutive patients, aged 19-60 years, with parenchymal sialadenitis associated with Sjogren's Syndrome, and 20 healthy donors. The exclusion criteria were exacerbation of sialadenitis, chronic infections, malignant neoplasms, and lymphoproliferative diseases. The standard diagnostic tests were minor salivary gland biopsy and parotid sialography. Immunophenotyping of parotid secretion lymphocytes was performed by multicolor flow cytometry. Lymphocytes were detectable in parotid secretion of patients affected by Sjogren's Syndrome, both primary (pSS) and secondary (sSS) form, but not in that from healthy donors. Sensitivity, specificity, positive, and negative predictive values of lymphocytes detection in parotid saliva were 77.5%, 100%, 100%, and 69%, respectively. The mean numbers of the total T-cell population, T-helper cells, and T-cytotoxic cells were 71.7%, 41.6%, and 53%, respectively. The immunophenotype of lymphocytes obtained by patients' parotid flow resembles the immunophenotypes of glandular biopsies currently known. Our preliminary data suggest the use of saliva as an alternative and non-invasive method for evaluating the prognosis of Sjogren's Syndrome.