Cytoprotective effects of paeoniflorin are associated with translocator protein 18 kDa
Cytoprotective effects of paeoniflorin are associated with translocator protein 18 kDa
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芍药苷的细胞保护作用与易位蛋白 18 kDa 相关
DOI：
10.1016/j.biopha.2018.07.112
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发表时间：
2018
影响因子：
7.5
通讯作者：
Chen Ji Sheng
中科院分区：
文献类型：
--
作者：
Qiu Zhi Kun;Liu Xu;Tang Dan;Zhang Zhe;Fan Qing Hong;Pan Yun Yun;Chen Ying Yu;Huang Mei Yan;Zhu Tao;Wang Yu Lu;Cheng Xiao Fang;Chen Ji Sheng
芍药苷是芍药中具有神经保护作用的重要活性成分之一。然而，有关脑星形胶质细胞的细胞保护因子尚不清楚。转运蛋白18 kDa（TSPO）及其下游的神经甾体生物合成在细胞保护中起重要作用。在此基础上，评价了TSPO和神经甾体生物合成在PF细胞保护作用中的作用。培养星形胶质细胞，选择AC-5216（TSPO配体）作为阳性对照药物。水溶性四氮唑法和酶联免疫吸附法分别定量测定PF的细胞保护作用和神经甾体水平。PF的细胞保护作用与神经甾体（如孕酮和别孕烯醇酮）的生物合成有关，而这些作用分别被PK 11195、曲洛司坦和非那肽完全阻断。因此，PF的细胞保护作用可能是通过TSPO和神经甾体的生物合成来实现的。这一发现为进一步研究PF的细胞保护作用提供了新的思路。
Paeoniflorin (PF) is one of the important active components in peony that are known to produce the neuroprotective effects. However, the involved cytoprotective factors on brain astrocytes are remain unclear. Translocator protein 18 kDa (TSPO) and its downstream neurosteroids biosynthesis play a significant role in cytoprotection. Based on these, the role of TSPO and neurosteroids biosynthesis in the cytoprotective effects of PF is evaluated. The astrocyte cells were cultured and AC-5216 (TSPO ligand) was selected as the positive control drug. The cytoprotective effects of PF and the levels of neurosteroids were quantified by water-soluble tetrazolium assay and enzyme linked immunosorbent assay, respectively. The cytoprotective activities of PF were relevant to neurosteroids (e.g. progsterone and allopregnanolone) biosynthesis, while these effects were totally blocked by PK11195, trilostane and finasteride, respectively. In summary, the cytoprotective effects of PF maybe mediated by TSPO and neurosteroids biosynthesis. The findings may provide the new insights into the cytoprotective effects of PF.