Reduction of Cr (VI) into Cr (III) by organelles of Chlorella vulgaris in aqueous solution: An organelle-level attempt
Reduction of Cr (VI) into Cr (III) by organelles of Chlorella vulgaris in aqueous solution: An organelle-level attempt
复制标题
水溶液中小球藻细胞器将 Cr (VI) 还原为 Cr (III)：细胞器水平的尝试
DOI：
10.1016/j.scitotenv.2016.07.217
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发表时间：
2016
影响因子：
9.8
通讯作者：
Wen Yuezhong
中科院分区：
文献类型：
--
作者：
Chen Zunwei;Song Shufang;Wen Yuezhong
优先污染物铬（Cr）是普遍存在的，为了方便获得和低成本，人们一直在努力通过生物降解将Cr（VI）还原为毒性较小的Cr（III）。然而，细胞器内的藻类细胞在Cr（VI）的还原功能的作用知之甚少。本研究提取了绿色藻小球藻（Chlorella vulgaris）的细胞器，并对其进行了修饰，用于Cr（VI）的还原试验。结果表明，叶绿体不仅对总铬有吸附能力（相对于对照为21.18%），而且对Cr（VI）有还原能力（相对于对照约为70%），其最适工作浓度为17 μg/mL。此外，分离的类囊体膜（ITM）显示出更好的Cr（VI）的还原电位与海藻酸钠（SA）的存在下，即使希尔反应活性（HRA）的抑制。对于光系统II（PSII），介孔氧化硅SBA-15的加入通过提高捕光复合物（LHC）II的效率和电子传递速率，增强了PSII的还原能力。从总体上看，C.普通小麦的PSII@SBA-15 >叶绿体> ITM@SA。本研究尝试用C还原Cr（VI）。这些细胞器不仅为探讨C还原Cr（VI）的潜在作用机制提供了基础。也为生物材料吸附重金属提供了新的视角。
The priority pollutant chromium (Cr) was ubiquitous and great efforts have been made to reduce Cr (VI) into less-toxic Cr (III) by alga for the convenient availability and low expense. However, the functional role of organelle inside the algal cell in Cr (VI) reduction was poorly understood. In this study, organelles in green algaeChlorella vulgariswere extracted and further decorated for Cr (VI) reduction tests. Results showed that the chloroplast exhibited not only adsorption ability of total Cr (21.18% comparing to control) but also reduction potential of Cr (VI) (almost 70% comparing to control), whose most suitable working concentration was at 17 μg/mL. Furtherly, the isolated thylakoid membrane (ITM) showed better Cr (VI) reduction potential with the presence of sodium alginate (SA), even though the Hill reaction activity (HRA) was inhibited. As for photosystem II (PSII), the addition of mesoporous silica SBA-15 enhanced the reduction ability through improving the light-harvesting complex (LHC) II efficiency and electron transport rate. On the whole, the reduction ability order of the three kinds of materials based on chloroplast inC. vulgariswas PSII@SBA-15 > Chloroplast > ITM@SA. The attempt made in this study to reduce the Cr (VI) withC. vulgarisorganelles might not only offer basement to detect the potential action mechanism of Cr (VI) reduction byC. vulgarisbut also provide a new sight for the scavenge of heavy metal with biological materials.