Development of miniaturized in situ analysis devices for biological and chemical oceanography
Development of miniaturized in situ analysis devices for biological and chemical oceanography
复制标题
DOI：
10.1109/mmb.2005.1548383
复制
发表时间：
2005-05
期刊：
影响因子：
--
通讯作者：
T. Fukuba;A. Imhof;M. Matsunaga;N. Takagi;T. Yamamoto;K. Okamura;T. Naganuma;T. Fujii
中科院分区：
文献类型：
--
作者：
T. Fukuba;A. Imhof;M. Matsunaga;N. Takagi;T. Yamamoto;K. Okamura;T. Naganuma;T. Fujii
本课题组研制了各种小型化、集成化的生物和化学海洋原位装置。介绍了用于海水微生物基因、重金属离子（锰：M/sup 2+/）浓度和pH值分析的装置。流通PCR聚合酶链反应）可以用具有集成加热和泵送系统的微生物基因分析装置进行。Mn/sup 2+/离子浓度通过重金属离子分析装置应用化学发光反应来定量。将微型泵集成在一起，开发出集成度高的pH测量装置。所有的器件都是基于PDMS（聚二甲基硅氧烷）微通道芯片与传统的成型方法制作的。
Various miniaturized and integrated in situ devices for biological and chemical oceanography have been developed in our research group. The devices targeted to analyze microbial gene, heavy metal ion (manganese: M/sup 2+/) concentration and pH of seawater are evaluated and presented here. Flow-through PCR polymerase chain reaction) can be performed with the microbial gene analysis device with integrated heating and pumping system. Mn/sup 2+/ ion concentration is quantified by applying chemiluminescence reaction by the heavy metal ion analysis device. Micropumps are integrated to develop well-integrated pH measurement device. All devices are based on PDMS (polydimethylsiloxane) microchannel chip fabricated with conventional molding method.