Probing cell surface interactions using atomic force microscope cantilevers functionalized for quantum dot-enabled Forster resonance energy transfer.
Probing cell surface interactions using atomic force microscope cantilevers functionalized for quantum dot-enabled Forster resonance energy transfer.
复制标题
使用原子力显微镜悬臂探测细胞表面相互作用，该悬臂可实现量子点福斯特共振能量转移。
DOI：
10.1117/1.3174429
复制
发表时间：
2009
影响因子：
3.5
通讯作者：
Meissner,KenithE
中科院分区：
文献类型：
--
作者：
Sun,Zhe;Juriani,Ameet;Meininger,GeraldA;Meissner,KenithE
首次报道了活细胞中量子点供体与红色荧光蛋白标记的整合素受体之间的Förster共振能量转移（FRET）。在二氧化硅微球表面涂上量子点，并将其固定在原子力显微镜（AFM）的悬臂梁上。然后将QD微球与纤连蛋白缀合以与HeLa细胞表面上表达的RFP-整联蛋白结合。在AFM控制的细胞与QD-微球结构接触之后，使用光漂白测量技术在QD-RFP对之间观察到FRET。这种FRET探针技术为生物医学和生物学研究中研究细胞表面受体-配体相互作用提供了一种新的工具。
Förster resonance energy transfer (FRET) between quantum dot (QD) donors and red fluorescent protein (RFP)-tagged integrin acceptors in live cells is reported for the first time. A silica microsphere was coated withQDs and mounted to the cantilever of an atomic force microscope (AFM). The QD microsphere is then conjugated with fibronectin to bind with RFP-integrins expressed on the surface of HeLa cells. Following AFM-controlled cell contact with the QD-microsphere structure, FRET is observed between the QD-RFP pair using a photobleaching measurement technique. This FRET probe technique provides a novel tool for studying the cell surface receptor-ligand interactions in biomedical and biological research.