A novel technique to diagnose non-melanoma skin cancer by thermal conductivity measurements: Correlations with cancer stromal factors
A novel technique to diagnose non-melanoma skin cancer by thermal conductivity measurements: Correlations with cancer stromal factors
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DOI：
10.1111/exd.13997
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发表时间：
2019-08-12
影响因子：
3.6
通讯作者：
Aiba, Setsuya
中科院分区：
文献类型：
--
作者：
Fujimura, Taku;Okabe, Takahiro;Aiba, Setsuya
皮肤表面温度反映了人体的生理状态。基于有效导热系数(ETC)的准确测量皮肤癌的定量诊断方法是在手术治疗前区分非侵袭性和侵袭性黑色素瘤的有前途的诊断工具之一。为了验证这些发现，在本报告中，通过测量皮肤表面温度和皮肤等的绝对值，进一步测试了侵袭性和非侵袭性乳房外Paget病(EMPD)和鳞状细胞癌(SCC)的诊断方法。此外，为了探讨可能影响ETC的间质因素，进行了LL37、Periostin(POSTN)、MMP12和MMP28的免疫组织化学染色。浸润性鳞癌和EMPD组的皮肤表面温度高于原位鳞癌组。与正常皮肤相比，非侵袭性EMPD组和鳞癌组的ETC值显著降低，而侵袭性EMPD组的ETC值显著升高。免疫组织化学染色显示侵袭性EMPD和SCC中LL37产生细胞的百分比明显高于非侵袭性EMPD和SCC。Spearman等级相关检验显示，EMPD和SCC中MMP12阳性细胞百分率与ETC表达水平呈显著负相关(r=-.5997)。本研究提示ETC差值可作为非黑色素瘤皮肤癌的一种新的高准确率的诊断技术，特别是对于检测皮肤鳞癌和EMPD的真皮侵袭。
The skin surface temperature reflects the physiological state of the human body. Quantitative methods of identification of skin cancers based on accurate measurement of effective thermal conductivity (ETC) are among the promising diagnostic tools for differentiating non-invasive and invasive melanomas before surgical treatment. To validate these findings, in this report, the diagnostic methods for invasive and non-invasive extramammary Paget's disease (EMPD) and squamous cell carcinoma (SCC) were further tested by measuring the absolute value of skin surface temperature and the ETC of the skin. In addition, to investigate the stromal factors that might affect ETC, immunohistochemical staining for LL37, periostin (POSTN), MMP12, and MMP28 was performed. The invasive SCC and EMPD group showed a relatively higher skin surface temperature compared to the in situ SCC group. The non-invasive EMPD and SCC group showed significantly lower values of ETC at lesions, whereas the invasive EMPD group showed significantly higher ETC values at lesions compared to healthy skin. Immunohistochemical staining showed that the percentage of LL37-producing cells was significantly increased in invasive EMPD and SCC compared to that in non-invasive EMPD and SCC. Moreover, Spearman's rank correlation test showed a significant inverse correlation between the percentage of MMP12-positive cells and increased levels of ETC-expressing areas in EMPD and SCC (r = -.5997). The present study suggested that differences in ETC could be a novel high-accuracy diagnostic technique for non-melanoma skin cancer, especially for detecting dermal invasion of SCC and EMPD.