Screening of the rice viviparous mutants generated by endogenous retrotransposon Tos17 insertion.: Tagging of a zeaxanthin epoxidase gene and a novel OsTATC gene
Screening of the rice viviparous mutants generated by endogenous retrotransposon Tos17 insertion.: Tagging of a zeaxanthin epoxidase gene and a novel OsTATC gene
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DOI：
10.1104/pp.125.3.1248
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发表时间：
2001-03-01
期刊：
影响因子：
7.4
通讯作者：
Hirochika, H
中科院分区：
文献类型：
--
作者：
Agrawal, GK;Yamazaki, M;Hirochika, H
水稻反转录转座子Tos 17是植物中为数不多的具有活性的反转录转座子之一，其转座可通过组织培养激活。在这里，我们提出的组织培养诱导水稻胎生突变体的特性，以证明使用反转录转座子Tos 17作为内源插入诱变剂和克隆的标签基因的正向遗传学在解开基因功能的可行性。两个突变体被证明是由插入Tos 17引起的。Osaba 1是一个强胎生的枯萎型突变体，表现出低脱落酸水平，干旱后脱落酸水平几乎没有进一步增加。该突变体被证明是玉米黄质的环氧化受损。而表型较弱的突变体Ostatc在干旱条件下表现为淡绿色表型，脱落酸含量略有增加。Osaba 1和Ostalc的致病基因的推导的氨基酸表现出显着的高度同源性与玉米黄质eposidase分离自其他植物物种和与细菌Sec-独立的移位酶TATC。蛋白质，分别。这是转座子标签在水稻中的第一个例子。
The rice (Oryza sativa) retrotransposon Tos17 is one of a few active retrotransposons in plants and its transposition is activated by tissue culture. Here, we present the characterization of viviparous mutants of rice induced by tissue culture to demonstrate the feasibility of the use of retrotransposon Tos17 as an endogenous insertional mutagen and cloning of the tagged gene for forward genetics in unraveling the gene function. Two mutants were shown to be caused by the insertion of Tos17. Osaba1, a strong viviparous mutant with wilty phenotype, displayed low abscisic acid level and almost no further increase in its levels upon drought. The mutant is shown to be impaired in the epoxidation of zeaxanthin. On the other hand, Ostatc, a mutant with weak phenotype, exhibited the pale green phenotype and slight increase abscisic acid levels upon drought. Deduced amino acids of the causative genes of Osaba1 and Ostalc manifested a significantly high homology with zeaxanthin eposidase isolated from other plant species and with bacterial Sec-independent translocase TATC. protein, respectively. This is the first example of trasposon tagging in rice.