Sequencing of a 4.3 kbp region of chromosome 2 of Candida albicans reveals the presence of homologues of SHE9 from Saccharomyces cerevisiae and of bacterial phosphatidylinositol-phospholipase C
Sequencing of a 4.3 kbp region of chromosome 2 of Candida albicans reveals the presence of homologues of SHE9 from Saccharomyces cerevisiae and of bacterial phosphatidylinositol-phospholipase C
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作者：
Andaluz, E;Coque, JJR;Larriba, G
已测定了白色念珠菌LIG 4基因下游4.3 kb片段的核苷酸序列。该片段含有两个完整的ORF（ORF 1和ORF 2）和一个截短的ORF（ORF 3）。ORF 1（1029 bp; EMBL数据库，登录号AJ 277539）编码一个343个氨基酸的推定蛋白，其与细菌来源的磷脂酰肌醇特异性磷脂酶C（PI-PLC）具有高度相似性，并且与锥虫、蝇和人的相似蛋白具有较小程度的相似性。分离的ORF 1赋予大肠杆菌转化体PI-PLC活性，ORF 2（1572 bp; EMBL数据库，登录号AJ 277538）预测了一种524个氨基酸的蛋白质，其沿着大部分全长与来自酿酒酵母的Ydr 393 w具有高度相似性。该蛋白携带与不同来源的几种细胞骨架蛋白具有显著相似性的结构域，YDR 393 w（SHE 9）是孤儿基因，其过表达损害细胞生长。ORF 3似乎编码高度保守的蛋白酶体26 S调节亚基的同源物。版权所有（C）2001约翰威利父子有限公司
The nucleotide sequence of a 4.3 kb fragment downstream of the LIG4 gene of Candida albicans has been determined. This fragment contains two entire ORFs (ORF1 and ORF2) and a truncated one (ORF3), ORF1 (1029bp; EMBL databank, Accession No. AJ277539) encodes a putative protein of 343 amino acids with a high degree of similarity to phosphatidylinositol-specific phospholipases C (PI-PLC) of bacterial origin and, to a lesser degree, to similar proteins from trypanosome, fly and human. Isolated ORF1 confers PI-PLC activity to Escherichia coli transformants, ORF2 (1572 bp; EMBL databank, Accession No. AJ277538) predicts a protein of 524 amino acids with high similarity along most of the entire length to Ydr393w from Saccharomyces cerevisiae. This protein carries a domain with significant similarity to several cytoskeleton proteins of different origins, YDR393w (SHE9) is an orphan gene whose overexpression compromises cell growth. ORF3 appears to encode the homologue of the well-conserved proteasomal 26S regulatory subunit. Copyright (C) 2001 John Wiley & Sons, Ltd.