In vitro and in vivo magnetic resonance imaging (MRI) detection of GFP through magnetization transfer contrast (MTC)
In vitro and in vivo magnetic resonance imaging (MRI) detection of GFP through magnetization transfer contrast (MTC)
复制标题
DOI：
10.1016/j.neuroimage.2009.12.111
复制
发表时间：
2010-04-01
期刊：
影响因子：
5.7
通讯作者：
Pautler, Robia G.
中科院分区：
文献类型：
--
作者：
Perez-Torres, Carlos J.;Massaad, Cynthia A.;Pautler, Robia G.
绿色荧光蛋白（GFP）是一种广泛使用的基因表达分子标记。然而，由于光学成像的组织穿透性限制，其在体内成像中的使用仅限于透明组织。在这里，我们报告了一种利用磁化转移对比（MTC）磁共振成像（MRI）检测 GFP 的新方法。 MTC 是一种 MRI 方法，目前用于检测大分子变化，例如髓磷脂减少和胶原蛋白含量增加。我们采用 MTC MRI 成像在体外和体内小鼠模型中检测 GFP。我们证明了我们的方法产生的值是蛋白质特异性的且依赖于浓度。这种方法提供了一种灵活的、非侵入性的体内分子 MRI 成像策略，该策略取决于 GFP 报告基因的存在和浓度。 (C) 2009 Elsevier Inc. 保留所有权利。
Green fluorescent protein (GFP) is a widely utilized molecular marker of gene expression. However, its use in in vivo imaging has been restricted to transparent tissue mainly due to the tissue penetrance limitation of optical imaging. Here, we report a novel approach to detect GFP with Magnetization transfer contrast (MTC) magnetic resonance imaging (MRI). MTC is an MRI methodology currently utilized to detect macromolecule changes such as decrease in myelin and increase in collagen content. We employed MTC MRI imaging to detect GFP both in vitro and in in vivo mouse models. We demonstrated that our approach produces values that are protein specific, and concentration dependent. This approach provides a flexible, non-invasive in vivo molecular MRI imaging strategy that is dependent upon the presence and concentration of the GFP reporter. (C) 2009 Elsevier Inc. All rights reserved.