Osteoconductive bio-based meshes based on poly(hydroxybutyrate-co-hydroxyvalerate) and poly(butylene adipate-co-terephthalate) blends.
Osteoconductive bio-based meshes based on poly(hydroxybutyrate-co-hydroxyvalerate) and poly(butylene adipate-co-terephthalate) blends.
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DOI：
10.1016/j.msec.2014.01.047
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发表时间：
2014-05
期刊：
影响因子：
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通讯作者：
M. Nar;Gerrit Staufenberg;B. Yang;Lesli Robertson;R. H. Patel;V. Varanasi;N. D'Souza
中科院分区：
文献类型：
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作者：
M. Nar;Gerrit Staufenberg;B. Yang;Lesli Robertson;R. H. Patel;V. Varanasi;N. D'Souza
聚己二酸丁酯-对苯二甲酸酯（PBAT）和聚羟基丁酸酯-羟基戊酸酯（PHBV）是具有骨愈合应用潜力的生物聚合物。在这项研究中，假设聚合物混合物具有综合强度和骨导电性来支持成骨细胞胶原的形成。将PBAT （PBAT 100）和含20% PHBV的共混物（PBAT 80）挤压成纤维，然后编织成网孔。在经向和纬向测试了这些材料的拉伸性能；结果表明，纬纱方向比经纱方向具有更高的性能。单个纤维在磷酸盐缓冲盐水（PBS）中保存8周，并使用动态力学分析仪（DMA）测试存储和损耗模量。结果表明：机械松弛强度先减小后增大；体外骨传导性研究采用分化成骨细胞（MC3T3-E1亚克隆4细胞）进行。环境扫描电镜（ESEM）结果显示，α-MEM预浸泡2周后，细胞附着生长良好。用x射线衍射（XRD）测定了聚合物在体外降解两周后的结构变化。拉曼光谱显示，所有支架都支持支架表面形成胶原网络。对于针织织物制造、机械性能和骨导电性的结合，混纺提供了一条有效的途径。
Poly(butylene adipate-co-terephthalate) (PBAT) and Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) are biopolymers that have the potential to be used in applications of bone healing. In this study, it is hypothesized that the polymer blend has the combined strength and osteoconductivity to support osteoblast collagen formation. PBAT (PBAT 100), and a blend with 20% PHBV (PBAT 80) were extruded in the form of fibers and then knitted in the form of mesh. These were tested in the warp as well as weft direction for the tensile properties; these showed that the weft direction had higher performance than the warp. The individual fibers were kept in phosphate buffered saline (PBS) over the period of 8 weeks and were tested for the storage and loss modulus using a dynamic mechanical analyser (DMA). The results indicated that mechanical relaxation strength showed a decrease and then an increase. In vitro osteoconductivity studies were done by using differentiating osteoblasts (MC3T3-E1 subclone 4 cells). Environmental Scanning Electron Microscopy (ESEM) showed that pre-soaking the samples in α-MEM for two weeks resulted in cell attachment and growth. X-ray diffraction (XRD) was used to determine the change in structure of polymers due to in vitro degradation for two weeks. Raman spectroscopy showed that all scaffolds supported the formation of a collagenous network over the scaffold surfaces. For a combination of knittable manufacturing, mechanical performance and osteoconductivity, blends offer an effective route.