Evidence that arachidonic acid derived from neutrophils and prostaglandin E2 are associated with the induction of acute lung inflammation by lipopolysaccharide of Escherichia coli
Evidence that arachidonic acid derived from neutrophils and prostaglandin E2 are associated with the induction of acute lung inflammation by lipopolysaccharide of Escherichia coli
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DOI：
10.1007/s00011-004-1308-7
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发表时间：
2004-12-01
影响因子：
6.7
通讯作者：
Sannomiya, P
中科院分区：
文献类型：
--
作者：
Alba-Loureiro, TC;Martins, EF;Sannomiya, P
目的：探讨花生四烯酸（AA）和前列腺素E2（PGE 2）在大肠杆菌（E.材料：成年雄性Wistar大鼠，采用大肠杆菌内毒素（LPS）诱导的急性肺损伤模型。对于体外研究，如下所述，使用大鼠嗜中性粒细胞、支气管肺泡灌洗（BAL）液和肺血管内皮细胞。BAL中TNF-α、IL-1 β、LTB 4和PGE（2）的酶联免疫测定（ELISA）分析，LPS刺激后6 h取肺组织行血管内皮细胞ICAM-1免疫组化检测。结果：LPS灌注大鼠BAL中中性粒细胞数量增加了63倍（从0.5 × 10（6）到31.5 × 10（6）细胞），伴随着TNF-α和IL-1 β水平的增加（p < 0.001），血管内皮细胞ICAM-1表达增加了3倍。血中性粒细胞AA含量降低50%，BALF中PGE（2）含量升高3.5倍，而LTB 4含量无明显变化。结论：AA和PGE（2）与LPS刺激有关。
Objective: The involvement of arachidonic acid (AA) and PGE(2) during the E. coli lipopolysaccharide (LPS)-induced acute lung injury was investigated.Material: Adult male Wistar rats were used. For in vitro studies, rat neutrophils, bronchoalveolar lavage (BAL) fluid, and lung vascular endothelium were used, as described below.Treatment: Rats were given an intratracheal injection of LPS (750 mug).Methods: Total and differential cell counts in BAL fluid; enzyme-linked immunoassay (ELISA) analyses of TNF-alpha, IL-1beta, LTB4 and PGE(2) in BAL, and immunohistochemical detection of ICAM-I on lung vascular endothelium were performed six h after LPS challenge. Fatty acid composition of blood neutrophils and plasma was analyzed by HPLC.Results: Rats instilled with LPS presented a sixty three-fold increase in the number of neutrophils in BAL (from 0.5 x 10(6) to 31.5 x 10(6) cells), accompanied by increased levels of TNF-alpha and IL-1beta (p < 0.001), and a three-fold increase in ICAM-1 expression on vascular endothelium. The content of AA in blood neutrophils was reduced by 50%, whereas the level of PGE(2) in BAL was increased by 3.5 fold, without changes in the levels of LTB4.Conclusions: These findings suggest that AA and PGE(2) are associated with LPS challenge.