Successful Vitrification of In vivo Embryos Collected from Superovulated Japanese Black Cattle (Wagyu)
Successful Vitrification of In vivo Embryos Collected from Superovulated Japanese Black Cattle (Wagyu)
复制标题
成功玻璃化冷冻从超排卵日本黑牛（和牛）体内采集的胚胎
DOI：
10.1111/rda.12674
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发表时间：
2016-04-01
影响因子：
1.7
通讯作者：
Du, F.
中科院分区：
文献类型：
--
作者：
An, L.;Ling, P. P.;Du, F.
内容 本研究的目的是确定玻璃化冷冻对于日本黑牛（Wagyu）体内衍生胚胎（在超数排卵治疗和胚胎移植（MOET）计划后收集）是否是一种有效的方法。人工授精后第 7 天收集的体内来源的桑葚胚和囊胚，使用改良的液滴玻璃化 (MDV) 程序进行玻璃化，随后加热以转移 (ET) 到同步受体中。新鲜胚胎和使用标准化慢速冷冻程序（直接解冻/直接移植，DT）冷冻保存的胚胎作为 ET 对照。两种不同的促卵泡激素 (FSH) 来源，即加拿大促卵泡激素 (FSH BAH，24 名供体) 和中国科学院制备的品牌 (FSH CAS，16 名供体)，按照完善的 FSH 给药方案对一系列超排结果进行了比较。经过数据分析，两种 FSH 来源之间在胚胎冲洗时记录的排卵总数（10.5 vs 8.5；p=0.28）和可移植胚胎总数（6.2 vs 5.1；p=0.52）相似。 MDV ET（39.7%，n=78）、DT（35.2%，n=71）和新鲜对照（47.1%，n=34）产生相似的妊娠率（p>0.05）。当使用 MDV 时，与 DT 对应物 (24.3%) 相比，玻璃化桑葚的移植导致更高的妊娠率 (42.6%) (p=0.05)。与玻璃化囊胚移植相比，玻璃化桑椹胚移植也导致更高的妊娠率（42.6% vs. 29.4%；p
Content The aim of this study was to determine whether vitrification is an effective method when used for Japanese Black Cattle (Wagyu) invivo-derived embryos, collected following a superovulation treatment and embryo transfer (MOET) programme. In vivo-derived morula and blastocysts collected on day 7 after artificial insemination, were vitrified using a modified droplet vitrification (MDV) procedure and subsequently warmed for transfer (ET) into synchronized recipients. Fresh embryos, and embryos cryopreserved using a standardized slow freezing procedure (direct thaw/direct transfer, DT) served as ET controls. Two different follicle-stimulating hormone (FSH) sources, Folltropin((R)) Canada (FSH BAH, 24 donors) and a brand prepared by the Chinese Academy of Science (FSH CAS, 16 donors), were compared in a series of superovulation outcomes following well-established FSH administration protocols. Following data analysis, the total number of ovulations recorded at the time of embryo flushing (10.5 vs 8.5; p=0.28) and the total number of transferable embryos (6.2 vs 5.1; p=0.52) were similar between the two FSH sources. ET for MDV (39.7%, n=78), DT (35.2%, n=71) and fresh controls (47.1%, n=34) resulted in similar pregnancy rates (p>0.05). When MDV was used, a higher pregnancy rate (42.6%) resulted from the transfer of vitrified morulae, when compared to the DT counterparts (24.3%), (p=0.05). Transfer of vitrified morulae resulted also in higher pregnancy rate, when compared to the transfer of vitrified blastocysts (42.6% vs. 29.4%; p