Effects of radical scavengers and antioxidant on ischemic acute renal failure in rabbits.
Effects of radical scavengers and antioxidant on ischemic acute renal failure in rabbits.
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自由基清除剂和抗氧化剂对兔缺血性急性肾衰竭的影响。
DOI：
10.3109/08860229909066965
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发表时间：
1999
期刊：
影响因子：
3
通讯作者：
Y. K. Kim
中科院分区：
文献类型：
--
作者：
Su Yung Kim;C. Kim;H. Yoo;Y. K. Kim
本研究旨在探讨活性氧（ROS）是否参与家兔缺血性急性肾功能衰竭（IARF）的发病机制。双侧肾动脉夹持60 min诱导肾缺血。采用黄嘌呤氧化酶抑制剂（别嘌呤醇）、过氧化氢清除剂（过氧化氢酶）和羟基自由基清除剂（苯甲酸钠）联合预处理动物。缺血后24 h血清肌酐水平显著升高，并在72 h保持较高水平。缺血导致GFR降低，FENa升高。这些变化在清除剂预处理后明显减弱。缺血后72小时再血流后，肾脏皮质切片中对氨基棘皮酸的摄取和微粒体Na(+)-K(+)- atp酶活性均被抑制，表明肾小管运输功能受损，清道夫治疗显著减弱了这一功能。回流后72 h肾血流量明显减少，经清道夫预处理后恢复。当动物被强效抗氧化剂DPPD预处理时，皮质和髓质的脂质过氧化被显著抑制。然而，抗氧化剂的预处理并没有减轻缺血引起的肾功能损害。这些结果表明，自由基清除剂可能通过其他作用而不是清除ROS来发挥对缺血急性肾功能衰竭的保护作用。因此，数据不支持ROS参与家兔IARF。
This study was undertaken to determine whether reactive oxygen species (ROS) are involved in the pathogenesis of ischemic acute renal failure (IARF) in rabbits. Renal ischemia was induced by clamping bilateral renal arteries for 60 min. Animals were pretreated with combination of xanthine oxidase inhibitor (allopurinol), hydrogen peroxide scavenger (catalase), and hydroxyl radical scavenger (sodium benzoate). Serum creatinine level significantly increased 24 h after ischemia and remained higher to 72 h. Ischemia caused a reduction of GFR and an increase of FENa. Such changes were significantly attenuated by scavenger pretreatment. The uptake of p-aminohippurate in cortical slices and microsomal Na(+)-K(+)-ATPase activity were depressed in kidneys subjected to 72 h of reflow following ischemia, indicating impairment of tubular transport function, which were significantly attenuated by scavenger treatment. Renal blood flow 72 h after reflow was markedly reduced and it was restored by scavenger pretreatment. When animals were pretreated with a potent antioxidant DPPD, lipid peroxidation in cortex and medulla was significantly inhibited. However, ischemia-induced impairment of renal function was not attenuated by pretreatment of the antioxidant. These results suggest that radical scavengers may exert a protective effect against ischemia acute renal failure by other actions rather than ROS scavenging. Thus, the data do not support involvement of ROS in IARF in rabbits.
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DOI：
10.1152/ajprenal.1988.255.3.f450
发表时间：
1988
期刊：
The American journal of physiology
影响因子：
--
作者：
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DOI：
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1989
期刊：
The American journal of physiology
影响因子：
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作者：
Zager,RA;Gmur,DJ
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DOI：
10.1152/ajprenal.1987.253.4.f685
发表时间：
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期刊：
The American journal of physiology
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DOI：
--
发表时间：
1990
期刊：
The Journal of laboratory and clinical medicine
影响因子：
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作者：
Thornton,MA;Zager,RA
通讯作者：
Zager,RA
影响因子：
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作者：
Sheridan, AM;Fitzpatrick, S;Lieberthal, W
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