Overexpression of yeast homologs of the mammalian checkpoint gene RCC1 suppresses the class of alpha-tubulin mutations that arrest with excess microtubules.
Overexpression of yeast homologs of the mammalian checkpoint gene RCC1 suppresses the class of alpha-tubulin mutations that arrest with excess microtubules.
复制标题
哺乳动物检查点基因 RCC1 的酵母同源物的过度表达会抑制因过量微管而停滞的 α-微管蛋白突变。
DOI：
10.1093/genetics/137.2.381
复制
发表时间：
1994
期刊：
影响因子：
3.3
通讯作者：
Solomon,F
中科院分区：
文献类型：
--
作者：
Kirkpatrick,D;Solomon,F
真核细胞中的微管参与多种核质结构，反映细胞的功能需求和细胞周期位置。我们正在研究酿酒酵母中微管组装和组织的细胞调控。我们筛选了当过度表达时抑制α-微管蛋白条件突变的生长表型的基因，这些突变在不允许的温度下用多余的微管阻止(2类突变)。在这里，我们描述了一种这样的抑制元件，称为ATS1(阿尔法微管蛋白抑制因子)。该基因的过度表达可以挽救所有2类突变的生长和微管表型，但不能挽救没有微管的冷敏突变(1类突变)。ATS1的缺失赋予了一种适度的缓慢生长表型，在同时含有ATS1缺失和2类tub 1突变的菌株中略有增强。预测的ATS1蛋白由333个氨基酸组成，与哺乳动物有丝分裂控制基因RCC1和酿酒酵母基因SRM1/PRP20的产物具有相当大的结构同源性。SRM1/PRP20的过表达也抑制了2类突变。结果表明，该基因家族可能参与了微管和细胞周期之间的调节相互作用。
Microtubules in eukaryotic cells participate in a variety of nuclear and cytoplasmic structures, reflecting functional requirements and cell cycle position. We are studying the cellular regulation of microtubule assembly and organization in the yeast Saccharomyces cerevisiae. We screened for genes that when overexpressed suppress the growth phenotype of conditional mutants in alpha-tubulin that arrest with excess microtubules at the nonpermissive temperature (class 2 mutations). Here we describe one such suppressing element, called ATS1 (for Alpha Tubulin Suppressor). Overexpression of this gene rescues both the growth and microtubule phenotypes of all class 2 mutations, but not the cold-sensitive mutations that arrest with no microtubules (class 1 mutations). Deletion of ATS1 confers a modest slow growth phenotype which is slightly enhanced in strains containing both a deletion of ATS1 and a class 2 tub 1 mutation. The predicted ATS1 protein contains 333 amino acids and has considerable structural homology to the products of both the mammalian mitotic control gene RCC1 and the S. cerevisiae gene SRM1/PRP20. Overexpression of SRM1/PRP20 also suppresses class 2 mutants. The results suggest that this family of genes may participate in regulatory interactions between microtubules and the cell cycle.