De Novo Production of Metabolites by Fungal Co-culture of Trichophyton rubrum and Bionectria ochroleuca
De Novo Production of Metabolites by Fungal Co-culture of Trichophyton rubrum and Bionectria ochroleuca
复制标题
DOI：
10.1021/np400258f
复制
发表时间：
2013-06-01
影响因子：
5.1
通讯作者：
Wolfender, Jean-Luc
中科院分区：
文献类型：
--
作者：
Bertrand, Samuel;Schumpp, Olivier;Wolfender, Jean-Luc
真菌的共培养最近被描述为通过可能的基因激活诱导新代谢产物产生的有前途的策略。在固体培养基中真菌共培养物的大筛选已经确定了红色毛癣菌和Bionectria ochroleuca之间不寻常的长距离生长抑制。为了研究这种特定真菌相互作用中的代谢物诱导，对纯菌株培养物和它们的共培养物进行基于差示LC-MS的代谢物组学。通过对指纹图谱的比较，得到了5个新诱导的化合物，并利用软件半制备HPLC-MS对这些化合物进行了纯化。其中一个代谢产物被成功鉴定为4 "-hydroxysulfoxy-2，2"-dimethylthielavin P（3，5-dimethylorsellinic acid的取代三聚体）。在B的纯菌株培养物中发现了非硫酸化形式以及其他三种相关化合物。赭色白
The co-cultivation of fungi has recently been described as a promising strategy to induce the production of novel metabolites through possible gene activation. A large screening of fungal co-cultures in solid media has identified an unusual long-distance growth inhibition between Trichophyton rubrum and Bionectria ochroleuca. To study metabolite induction in this particular fungal interaction, differential LC-MS-based rnetabolomics was performed on pure strain cultures and on their co-cultures. The comparison of the resulting fingerprints highlighted five de novo induced compounds, which were purified using software-oriented semipreparative HPLC-MS. One metabolite Was successfully identified as 4 ''-hydroxysulfoxy-2,2 ''-dimethylthielavin P (a substituted trimer of 3,5-dimethylorsellinic acid). The nonsulfated form, as well as, three other related compounds, were found in the pure strain culture of B. ochroleuca.