The tricornered Ser/Thr protein kinase is regulated by phosphorylation and interacts with furry during Drosophila wing hair development
The tricornered Ser/Thr protein kinase is regulated by phosphorylation and interacts with furry during Drosophila wing hair development
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DOI：
10.1091/mbc.e04-09-0828
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发表时间：
2005-02-01
影响因子：
3.3
通讯作者：
Adler, PN
中科院分区：
文献类型：
--
作者：
He, Y;Fang, XL;Adler, PN
Ser/Thr激酶的Trc/Ndr/Saxi/Cbk 1家族在果蝇、蠕虫和酵母的极化细胞结构的形态发生中起关键作用。Tricornered（Trc）是果蝇核Dbf 2相关（Ndr）丝氨酸/苏氨酸蛋白激酶，是表皮毛、刚毛、毛细胞和树突的正常形态发生所必需的。我们在体内获得的证据表明，Trc的功能是由磷酸化和关键调控位点的突变导致显性负等位基因。我们发现，野生型，但不是突变的Trc，发现在生长的头发，我们未能检测到Trc蛹翅核，这意味着在这种发展背景下，Trc的功能在细胞质中。在酵母和秀丽隐杆线虫中的毛茸茸的基因及其同源物先前被认为是Ndr激酶家族功能的必需品。我们发现，果蝇毛皮（Fry）也被发现在生长的头发，它的亚细胞定位是依赖于Trc功能，它可以与Trc免疫共沉淀。我们的数据表明，涉及Trc活性的反馈机制调节Fry在发育中的毛发中的积累。
The Trc/Ndr/Saxi/Cbk1 family of ser/thr kinases plays a key role in the morphogenesis of polarized cell structures in flies, worms, and yeast. Tricornered (Trc), the Drosophila nuclear Dbf2-related (Ndr) serine/threonine protein kinase, is required for the normal morphogenesis of epidermal hairs, bristles, laterals, and dendrites. We obtained in vivo evidence that Trc function was regulated by phosphorylation and that mutations in key regulatory sites resulted in dominant negative alleles. We found that wild-type, but not mutant Trc, is found in growing hairs, and we failed to detect Trc in pupal wing nuclei, implying that in this developmental context Trc functions in the cytoplasm. The furry gene and its homologues in yeast and Caenorhabditis elegans have previously been implicated as being essenhal for the function of the Ndr kinase family. We found that Drosophila furry (Fry) also is found in growing hairs, that its subcellular localization is dependent on Trc function, and that it can be coimmunoprecipitated with Trc. Our data suggest a feedback mechanism involving Trc activity regulates the accumulation of Fry in developing hairs.