Transcriptome Analysis for Abnormal Spike Development of the Wheat Mutant dms.
Transcriptome Analysis for Abnormal Spike Development of the Wheat Mutant dms.
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小麦突变体 dms 穗发育异常的转录组分析
DOI：
10.1371/journal.pone.0149287
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Jiang YM
中科院分区：
文献类型：
--
作者：
Zhu XX;Li QY;Shen CC;Duan ZB;Yu DY;Niu JS;Ni YJ;Jiang YM
背景小麦（Triticum aestivum L.）穗部发育是籽粒产量的基础。获得了一个矮秆、多雌蕊、不育的小麦突变体dms。虽然遗传变化尚不清楚，但性状遗传表明，在中等株（M）的自花授粉群体中，多雌蕊和不育两个性状由一对隐性基因控制，因此，突变体后代中的矮秆基因型（D）和高秆基因型（T）是研究小麦穗发育的理想品系。本研究旨在探讨矮秆小麦穗部异常的分子基础。结果通过对D、T两种植物的超体积分化穗和茎尖的mRNA测序，构建了4个单基因文库。通过整合分析，我们鉴定了419个在穗中高表达的基因，包括MADS-box家族的9个典型同源异型基因和TaAP 2、TaFL和TaDL基因。我们还鉴定了143个在T和D幼穗间差异显著的基因，以及26个与幼穗分化有关的基因。结果表明，TaAP 1 -2、TaAP 2以及参与转录、翻译、细胞分裂、光合作用、碳水化合物转运和代谢、能量生产和转化等生物学过程的基因在D中的表达水平显著低于T。结论我们鉴定了一组与小麦花器官分化相关的基因，包括典型的同源异型基因。结果表明，同源异型基因的低表达、激素失衡、生物学过程受抑制以及结构材料和能量的缺乏是导致dms穗异常的主要原因。我们对dms的同源异型基因进行了一系列的研究，但对引起dms棘波异常表型的其他三个因素还需进一步研究。
Background Wheat (Triticum aestivum L.) spike development is the foundation for grain yield. We obtained a novel wheat mutant, dms, characterized as dwarf, multi-pistil and sterility. Although the genetic changes are not clear, the heredity of traits suggests that a recessive gene locus controls the two traits of multi-pistil and sterility in self-pollinating populations of the medium plants (M), such that the dwarf genotype (D) and tall genotype (T) in the progeny of the mutant are ideal lines for studies regarding wheat spike development. The objective of this study was to explore the molecular basis for spike abnormalities of dwarf genotype. Results Four unigene libraries were assembled by sequencing the mRNAs of the super-bulked differentiating spikes and stem tips of the D and T plants. Using integrative analysis, we identified 419 genes highly expressed in spikes, including nine typical homeotic genes of the MADS-box family and the genes TaAP2, TaFL and TaDL. We also identified 143 genes that were significantly different between young spikes of T and D, and 26 genes that were putatively involved in spike differentiation. The result showed that the expression levels of TaAP1-2, TaAP2, and other genes involved in the majority of biological processes such as transcription, translation, cell division, photosynthesis, carbohydrate transport and metabolism, and energy production and conversion were significantly lower in D than in T. Conclusions We identified a set of genes related to wheat floral organ differentiation, including typical homeotic genes. Our results showed that the major causal factors resulting in the spike abnormalities of dms were the lower expression homeotic genes, hormonal imbalance, repressed biological processes, and deficiency of construction materials and energy. We performed a series of studies on the homeotic genes, however the other three causal factors for spike abnormal phenotype of dms need further study.