Human ovarian epithelial cancer cells cultured in Vitro express both interleukin 1α and β genes
Human ovarian epithelial cancer cells cultured in Vitro express both interleukin 1α and β genes
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体外培养的人卵巢上皮癌细胞同时表达白细胞介素1α和β基因
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
S. Ramakrishnan
中科院分区：
文献类型：
--
作者：
Bing Li;D. Mohanraj;M. Olson;M. Moradi;L. Twiggs;L. Carson;S. Ramakrishnan
卵巢癌患者腹水中常含有大量的白细胞和肿瘤细胞。最近的一些证据表明，腹水中含有能够在体外和体内诱导卵巢癌细胞生长的因子。虽然这些因素还没有完全确定，但肿瘤细胞分泌的生长因子可以通过旁分泌和自分泌机制影响肿瘤的生长。早些时候，我们报道了卵巢上皮性癌细胞产生巨噬细胞集落刺激因子。这些肿瘤细胞似乎产生不止一种细胞因子。识别肿瘤细胞分泌的各种产物将提供了解卵巢癌生物学所需的有价值的信息。本研究首次提供了5个不同的人卵巢上皮性肿瘤细胞系和从4例癌症患者腹水中分离的肿瘤细胞组成表达白介素1α和β基因的证据。通过分析IL-1相关转录物的细胞RNA和免疫学分析来确定淋巴因子的产生。卵巢癌细胞还分泌另一种多效性细胞因子IL-6。在许多系统中，IL-1诱导IL-6基因的表达。为了确定IL-6产生的基础水平是否依赖于内源性IL-1，进行了中和研究。加入IL-1抗体并不能降低癌细胞分泌IL-6的水平。这些结果提示卵巢癌细胞产生多种细胞因子，内源性IL-1可能不直接参与这些细胞中IL-6基因表达的调节。
Ascitic fluid from human ovarian cancer patients often contains a large number of leukocytes along with tumor cells. Some of the recent evidence suggests that the ascitic fluid contains factors capable of inducing the growth of ovarian cancer cells in vitro and in vivo . While these factors have not yet been completely characterized, growth factors secreted by the tumor cells could influence the tumor growth by paracrine and autocrine mechanisms. Earlier, we reported that ovarian epithelial cancer cells produce macrophage colony-stimulating factor. It appears that these tumor cells produce more than one cytokine. Identifying the various products secreted by the tumor cells would provide valuable information needed to understand the biology of ovarian cancer. In the present study, evidence is provided for the first time that five different human ovarian epithelial tumor cell lines and tumor cells isolated from the ascitic fluid of four cancer patients express interleukin (IL) 1α and β genes constitutively. Production of the lymphokine was determined by analyzing the cellular RNA for IL-1-related transcripts and by immunological assays. Ovarian cancer cells also secrete another pleiotropic cytokine, IL-6, constitutively. In many systems, IL-1 induces the expression of the IL-6 gene. To determine whether the basal levels of IL-6 production are dependent on the endogenous IL-1, neutralization studies were carried out. Addition of antibodies to IL-1 did not decrease the levels of IL-6 secreted by the cancer cell lines. These results suggest that multiple cytokines are produced by ovarian cancer cells and that the endogenous IL-1 may not be directly involved in the regulation of IL-6 gene expression in these cells.
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影响因子：
11.2
作者：
A. Berchuck;R. Whitaker;G. Olt;J. Soper;D. Clarke‐Pearson;A. Kamel;A. Kamel;R. Bast;B. Kerns;R. Kinney;R. Dodge;P. Marks;S. McKenzie;S. Yin
通讯作者：
A. Berchuck;R. Whitaker;G. Olt;J. Soper;D. Clarke‐Pearson;A. Kamel;A. Kamel;R. Bast;B. Kerns;R. Kinney;R. Dodge;P. Marks;S. McKenzie;S. Yin
影响因子：
5.2
作者：
Ray,A;Tatter,SB;Santhanam,U;Helfgott,DC;May,LT;Sehgal,PB
通讯作者：
Sehgal,PB
人卵巢癌体内肿瘤坏死因子的表达。
影响因子：
11.2
作者：
Takeyama,H;Wakamiya,N;O'Hara,C;Arthur,K;Niloff,J;Kufe,D;Sakarai,K;Spriggs,D
通讯作者：
Spriggs,D
影响因子：
56.9
作者：
SLAMON, DJ;GODOLPHIN, W;PRESS, MF
通讯作者：
PRESS, MF
卵巢癌细胞系和原代卵巢肿瘤培养物组成型产生白细胞介素 6。
影响因子：
11.2
作者：
Watson,JM;Sensintaffar,JL;Berek,JS;Martinez-Maza,O
通讯作者：
Martinez-Maza,O