Diurnal variation in glutathione and cysteine redox states in human plasmas
Diurnal variation in glutathione and cysteine redox states in human plasmas
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DOI：
10.1093/ajcn/86.4.1016
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发表时间：
2007-10-01
影响因子：
7.1
通讯作者：
Jones, Dean P.
中科院分区：
文献类型：
--
作者：
Blanco, Roberto A.;Ziegler, Thomas R.;Jones, Dean P.
背景资料：血浆谷胱甘肽/谷胱甘肽二硫化物（GSH/GSSG）和半胱氨酸/胱氨酸（Cys/CySS）对在人体内被氧化，与氧化应激和心血管疾病风险相关。动物研究表明，这两个池经历与饮食摄入的含硫氨基酸相关的昼夜变化。目的：本研究的目的是确定健康成年人的GSH，Cys，GSH/GSSG或Cys/CySS的氧化还原状态是否经历昼夜变化。设计：63名18-86岁正常饮食（蛋白质，0.8%）的老年人，每小时采集一次血浆样品，共24小时。kg（-1）。d（-1）;含硫氨基酸，20 m . kg（-1）。d（-1））。结果：血浆Cys、GSH浓度随时间变化，Cys、GSH浓度随时间变化，Cys、GSH浓度随时间变化，Cys、GSH浓度随时间变化。血浆Cys的最高值出现在餐后约3 μ l。谷氨酰胺在血浆Cys峰值后6 h达到最大值。GSH/GSSG和Cys/CySS对的计算的氧化还原状态与硫醇形式的浓度相关。Cys/CySS对的最大还原和氧化发生在2130和0630，而GSH/GSSG对的相应值发生在0330和1330。年龄≥ 60岁的人Cys/CySS氧化还原的平均日变化是年龄≥ 60岁的人的1.8倍。
Background: Plasma glutathione/glutathione disulfide (GSH/GSSG) and cysteine/cystine (Cys/CySS) couples are oxidized in humans in association with oxidative stress and cardiovascular disease risk. Animal studies show that both pools undergo diurnal variations associated with dietary intake of sulfur amino acids.Objective: The objective of this study was to determine whether the redox state of GSH, Cys, GSH/GSSG, or Cys/CySS undergoes diurnal variation in healthy adults.Design: Plasma samples were collected every hour for 24 h from 63 persons aged 18-86 y who were consuming normal food (protein, 0.8 . kg(-1) . d(-1); sulfur amino acids, 20 m . kg(-1) . d(-1)) at standardized mealtimes. Measurements of Cys, CySS, GSH, and GSSG were used with the Nernst equation to calculate the redox states.Results: Plasma Cys and GSH concentrations varied with the time of day. The highest values for plasma Cys occurred approximate to 3 It after meals. Glutathione was maximal 6 h after peak plasma Cys. The calculated redox states of the GSH/GSSG and Cys/CySS couples varied in association with the concentrations of the thiol forms. Maximal reduction and oxidation of the Cys/CySS couple Occurred at 2130 and 0630, whereas the respective values for the GSH/GSSG couple occurred at 0330 and 1330. The mean diurnal variation for Cys/CySS redox in persons aged >= 60 y was 1.8-fold that in persons aged