Heterogeneity in thylakoid membrane proteome of Synechocystis 6803
Heterogeneity in thylakoid membrane proteome of Synechocystis 6803
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DOI：
10.1016/j.jprot.2009.12.011
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发表时间：
2010-03-10
影响因子：
3.3
通讯作者：
Sainis, Jayashree Krishna
中科院分区：
文献类型：
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作者：
Agarwal, Rachna;Matros, Andrea;Sainis, Jayashree Krishna
集胞藻6803的无细胞提取物通过40，000 xg、90，000 xg和150，000 xg的连续超离心分级，以获得命名为40 k的三个类囊体级分，这些级分在吸收和发射光谱中显示出差异。TOF MS分析鉴定了123个属于膜和胞质组分的蛋白质。有22个蛋白质具有跨膜螺旋，12个蛋白质具有信号肽。光合复合物、CF 0-CF 1 ATP合酶或核糖体蛋白质在其余蛋白质中，8个为40 k组分所独有，90 k和150 k组分间共有10种蛋白质，90 k和150 k组分间共有12种蛋白质，未检测到共有蛋白质结果表明集胞藻6803类囊体存在异质性，这可能导致该生物体类囊体的微区室化和功能异质性，如前所述（C）2009 Elsevier B.V保留所有权利
Cell-free extracts of Synechocystis 6803 were fractionated by successive ultracentrifugation at 40,000xg, 90,000xg and 150,000 x g to obtain the three thylakoid fractions designated as 40 k, 90 k and 150 k fractions respectively These fractions showed differences in absorption and emission spectra Nano-LC-ESI-Q-TOF MS analysis identified 123 proteins belonging to membrane as well as cytosolic fraction Out of these proteins, there were 22 proteins with transmembrane helices and 12 proteins with signal peptide There were 77 proteins common across all the three fractions Most of these proteins were subunits of photosynthetic complexes, CF0-CF1 ATP synthase or ribosomal proteins Among the rest of the proteins, 8 were exclusive to 40 k fraction, 3 were exclusive to 90 k fraction and 13 were exclusive to 150 k fraction There were 10 proteins common between 40 k and 90 k fractions and 12 proteins common between 90 k and 150 k fractions There were no common proteins detected between 40 k and 150 fractions The results suggested existence of heterogeneity in thylakoids of Synechocystis 6803, which may lead to micro-compartmentation and functional heterogeneity in the thylakoids of this organism as seen previously (C) 2009 Elsevier B.V All rights reserved