Sequencing and generation of an infectious clone of the pathogenic goose parvovirus strain LH
Sequencing and generation of an infectious clone of the pathogenic goose parvovirus strain LH
复制标题
致病性鹅细小病毒 LH 株感染性克隆的测序和产生
DOI：
10.1007/s00705-014-2319-5
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发表时间：
2015-03-01
影响因子：
2.7
通讯作者：
Zhu, Guoqiang
中科院分区：
文献类型：
--
作者：
Wang, Jianye;Duan, Jinkun;Zhu, Guoqiang
摘要：本研究对鹅细小病毒（GPV）强毒株LH的完整基因组进行了测序，并将其克隆到pBluescript II（SK）质粒载体中。 GPV 毒株反向末端重复序列 (ITR) 的序列比对显示，与早于该时间分离的三个 GPV 毒株相比，LH 毒株和 1982 年之后分离的其他三个毒株的回文结构茎中存在常见的 14 nt 对缺失。用含有 LH 株完整基因组的质粒 pLH 转染 11 天的鹅胚蛋，成功拯救了传染性病毒。通过绒毛尿囊膜浸润途径转染后胚胎死亡早于通过尿囊腔接种途径转染时发生。根据小鹅的死亡率评估，获救的病毒表现出与其亲代病毒相似的毒力。致病性感染性克隆的产生为我们将来阐明GPV的分子发病机制提供了有力的工具。
AbstractsIn this study, the complete genome of the virulent strain LH of goose parvovirus (GPV) was sequenced and cloned into the pBluescript II (SK) plasmid vector. Sequence alignments of the inverted terminal repeats (ITR) of GPV strains revealed a common 14-nt-pair deletion in the stem of the palindromic structure in the LH strain and three other strains isolated after 1982 when compared to three GPV strains isolated earlier than that time. Transfection of 11-day-old embryonated goose eggs with the plasmid pLH, which contains the entire genome of strain LH, resulted in successful rescue of the infectious virus. Death of embryos after transfection via the chorioallantoic membrane infiltration route occurred earlier than when transfection was done via the allantoic cavity inoculation route. The rescued virus exhibited virulence similar to that of its parental virus, as evaluated by the mortality rate in goslings. Generation of the pathogenic infectious clone provides us with a powerful tool to elucidate the molecular pathogenesis of GPV in the future.