Trophoblasts isolated from the maternal circulation: In vitro expansion and potential application in non-invasive prenatal diagnosis
Trophoblasts isolated from the maternal circulation: In vitro expansion and potential application in non-invasive prenatal diagnosis
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DOI：
10.1369/jhc.4b6426.2005
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发表时间：
2005-03-01
影响因子：
3.2
通讯作者：
Barkai, G
中科院分区：
文献类型：
--
作者：
Guetta, E;Gutstein-Abo, L;Barkai, G
基于母体血液中罕见胎儿细胞的产前诊断目前还不可行。通过靶向滋养层细胞来提高细胞产量。分选后直接或培养后分析hla - g阳性细胞部分。原位杂交技术被用于证明携带男性胎儿的女性样本中的胎儿细胞来源，并在之前不知道胎儿性别的情况下预测样本中的性别。体外培养导致93%的这些样本中胎儿细胞和准确的性别预测显著增加。这种方法可能对无创产前诊断有用。
Prenatal diagnosis based on rare fetal cells in maternal blood is currently not a feasible option. An effort was made to improve cell yields by targeting trophoblast cells. After sorting, the HLA-G-positive cell fraction was analyzed directly or after culture. In situ hybridization technology was applied to prove fetal cell source in samples from women carrying a male fetus and to predict gender in samples without previous knowledge of fetal sex. In vitro culture led to a significant increase in fetal cells and accurate gender prediction in 93% of these samples. This approach might be useful for non-invasive prenatal diagnosis.