ROLE OF OXYGEN DERIVED FREE-RADICALS IN PLATELET ACTIVATING FACTOR INDUCED BOWEL NECROSIS
ROLE OF OXYGEN DERIVED FREE-RADICALS IN PLATELET ACTIVATING FACTOR INDUCED BOWEL NECROSIS
复制标题
DOI：
10.1136/gut.29.9.1207
复制
发表时间：
1988-09-01
期刊：
影响因子：
24.5
通讯作者：
HSUEH, W
中科院分区：
文献类型：
--
作者：
CUEVA, JP;HSUEH, W
缺血性肠坏死的组织和细胞损伤机制尚不清楚。本研究通过向肠系膜血管注射血小板活化因子（PAF），探讨了氧自由基在肠坏死发展中的作用。在给予PAF之前，动物用别嘌呤醇或超氧化物歧化酶与过氧化氢酶一起预处理。SOD/CAT可明显改善PAF引起的肠道损伤，提示PAF引起的肠道损伤主要是氧自由基的释放所致。氧自由基的主要来源是黄嘌呤氧化酶，因为别嘌呤醇改善了小肠病变。别嘌呤醇预处理对PAF引起的低血压有明显的预防作用（P < 0.01）。相比之下，超氧化物歧化酶/过氧化氢酶并没有改变PAF诱导的低血压。超氧化物歧化酶/过氧化氢酶预处理可改善PAF引起的血液浓缩和白细胞减少，而allupurinol无此作用。
The mechanism of tissue and cell injury in ischaemic bowel necrosis is unclear. The present study investigated the role of oxygen derived free radicals in the development of bowel necrosis using injections of platelet activating factor (PAF) into the mesenteric vasculature. Animals were pretreated with allopurinol or superoxide dismutase together with catalase, before administration of PAF. Sueproxide dismutase/catalase markedly improved the PAF-induced lesions, indicating that most of the intestinal damage after PAF injection is because of the release of oxygen radicals. The major source of oxygen radicals is xanthine oxidase, as allopurinol ameliorated small bowel lesions. Pretreatment with allopurinol produced a significant (p < 0.01) preventive effect on PAF induced hypotension. In contrast, superoxide dismutase/catalase did not alter PAF induced hypotension. Superoxide dismutase/catalase pretreatmet improved PAF induced haemoconcentration and leucopenia, while allupurinol showed no effect.