Direct anticryptococcal activity of lymphocytes from Cryptococcus neoformans-immunized mice.
Direct anticryptococcal activity of lymphocytes from Cryptococcus neoformans-immunized mice.
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新生隐球菌免疫小鼠淋巴细胞的直接抗隐球菌活性。
DOI：
10.1128/iai.63.5.1637-1644.1995
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发表时间：
1995
影响因子：
3.1
通讯作者：
Murphy,JW
中科院分区：
文献类型：
--
作者：
Muth,SM;Murphy,JW
评估新隐球菌免疫小鼠和对照小鼠淋巴细胞的直接抗隐球菌活性是本研究的重点。我们证明，在效应细胞与隐球菌靶细胞比例为2:1时，由新隐球菌免疫小鼠的α β T细胞受体阳性T淋巴细胞（98%至99% CD3+）组成的效应细胞群比未免疫对照小鼠的相似淋巴细胞群更好地抑制隐球菌细胞的生长。在将隐球菌与效应细胞混合后，几乎立即观察到新生隐球菌与淋巴细胞结合。在孵育的前30分钟内，结合物的百分比增加，然后在接下来的1.5小时内保持不变。在评估的每个时间段，来自新隐球菌免疫小鼠的T淋巴细胞富集群体与对照T淋巴细胞形成的隐球菌结合物的百分比显著高于对照T淋巴细胞。为了抑制生长，效应细胞和靶细胞之间的直接接触是必要的，在生长抑制实验中，当淋巴细胞和隐球菌细胞群被多孔膜分离时，隐球菌生长抑制就被取消了。与T细胞富集群体孵育后的隐球菌生命染色显示T淋巴细胞杀死隐球菌细胞。
Assessment of the direct anticryptococcal activity of murine lymphocytes from both Cryptococcus neoformans-immunized and control mice was the focus of this investigation. We demonstrate that at a 2:1 effector cell-to-cryptococcal target cell ratio, effector cell populations comprised of alpha beta T-cell receptor-positive T lymphocytes (98 to 99% CD3+) from C. neoformans-immunized mice inhibited the growth of cryptococcal cells better than similar populations of lymphocytes from nonimmunized control mice. Almost immediately after mixing of cryptococci with the effector cells, C. neoformans-lymphocyte conjugates were observed. The percentage of conjugates increased over the first 30 min of incubation and then remained constant over the next 1.5 h. T-lymphocyte-enriched populations from C. neoformans-immunized mice formed significantly greater percentages of conjugates with cryptococci than control T lymphocytes at each time period that assessment was made. For growth inhibition to occur, direct contact between the effector and target cells was necessary, as evidenced by abrogation of cryptococcal growth inhibition when lymphocyte and cryptococcal cell populations were separated by a porous membrane during the growth inhibition assay. Vital staining of cryptococci after incubation with the T-cell-enriched populations showed that the T lymphocytes killed the cryptococcal cells.