Effects of Dorzolamide on Retinal and Choroidal Blood Flow in the DBA/2J Mouse Model of Glaucoma.
Effects of Dorzolamide on Retinal and Choroidal Blood Flow in the DBA/2J Mouse Model of Glaucoma.
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DOI：
10.1167/iovs.15-18291
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发表时间：
2016-03
影响因子：
4.4
通讯作者：
Duong TQ
中科院分区：
文献类型：
--
作者：
Chandra S;Muir ER;Deo K;Kiel JW;Duong TQ
在DBA/2 J小鼠青光眼模型中，检验急性外用多佐胺（DZ）降低眼内压（IOP）并增加视网膜和脉络膜血流量的假设。在异氟烷麻醉下，使用磁共振成像测量4月龄和9月龄DBA/2 J小鼠和4月龄C57 BL/6（对照）小鼠的视网膜和脉络膜血流。在基线和局部多佐胺给药后1小时和2小时测量眼血流量。在相同的时间点，使用回弹眼压计测量一组动物的眼内压。4月龄DBA/2 J小鼠和C57 BL/6小鼠的基线IOP无显著差异（P > 0.05），两组的IOP均低于9月龄DBA/2 J小鼠（均P < 0.05）。与基线相比，在4月龄（P < 0.05）和9月龄（P < 0.01）DBA/2 J小鼠中，多佐胺在给药后1小时和2小时降低IOP，但在C57 BL/6 J小鼠中未降低IOP（P > 0.05）。与4月龄C57 BL/6 J小鼠相比，4月龄和9月龄DBA/2 J小鼠的基线视网膜血流量较低（P < 0.05）。9月龄DBA/2 J小鼠的基线脉络膜血流量低于C57 BL/6 J小鼠（P < 0.05）。与基线相比，在9月龄DBA/2 J小鼠中，多佐胺给药后1小时视网膜和脉络膜血流量增加，2小时后仍保持升高（P < 0.05）。多佐胺可降低老年DBA/2 J小鼠的IOP并增加视网膜和脉络膜血流量，与研究假设一致。
To test the hypothesis that acute topical dorzolamide (DZ) decreases intraocular pressure (IOP) and increases retinal and choroidal blood flow in the DBA/2J mouse model of glaucoma. Retinal and choroidal blood flow were measured in 4- and 9-month-old DBA/2J mice, and 4-month C57BL/6 (control) mice under isoflurane anesthesia using magnetic resonance imaging. Ocular blood flow was measured at baseline, and 1 and 2 hours after topical dorzolamide. Intraocular pressure was measured using a rebound tonometer in a subset of animals at the same time points. Baseline IOP in the 4-month-old DBA/2J mice and C57BL/6 mice was not significantly different (P > 0.05), and IOP in both groups was less than in the 9-month-old DBA/2J mice (P < 0.05 for both). Compared to baseline, dorzolamide reduced IOP at 1 and 2 hours after dorzolamide in the 4- (P < 0.05) and 9-month-old (P < 0.01) DBA/2J mice, but not in the C57BL/6J mice (P > 0.05). Baseline retinal blood flow was lower in the 4-month and 9-month-old DBA/2J mice compared with the 4-month-old C57BL/6J mice (P < 0.05). Baseline choroidal blood flow in the 9-month-old DBA/2J mice was less than in the C57BL/6J mice (P < 0.05). Compared with baseline, both retinal and choroidal blood flow increased at 1-hour post-dorzolamide and remained elevated 2 hours later in the 9-month-old DBA/2J mice (P < 0.05). Dorzolamide lowers IOP and raises retinal and choroidal blood flow in older DBA/2J mice, consistent with the study hypothesis.