Comparison of gene expression responses to hypoxia in viviparous (Xiphophorus) and oviparous (Oryzias) fishes using a medaka microarray
Comparison of gene expression responses to hypoxia in viviparous (Xiphophorus) and oviparous (Oryzias) fishes using a medaka microarray
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DOI：
10.1016/j.cbpc.2008.11.005
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发表时间：
2009-03-01
影响因子：
3.9
通讯作者：
Walter, Ronald B.
中科院分区：
文献类型：
--
作者：
Boswell, Mikki G.;Wells, Melissa C.;Walter, Ronald B.
利用DNA微阵列技术进行基因表达谱分析是评估生物体暴露于环境胁迫后基因转录水平反应的有用工具。在这里，我们详细介绍了使用8k Medaka(Oryzias Latipe)微阵列的研究结果，以评估在低氧暴露下活着的水族鱼剑鱼的基因表达模式。为了评估在跨属杂交中使用青竹阵列的重复性和可靠性，对基因表达进行了双因素方差分析。数据显示，用于阵列杂交的RNA的组织来源比RNA的物种来源对观察到的调制基因靶标的反应贡献更大。此外，通过对组织和物种分组(剑鱼和青竹)的热图分析进行的等级聚类表明，低氧在这两种不同的水生模式生物的相同组织中诱导了类似的反应。我们的剑鱼实验结果表明，206个大脑、37个肝脏和925个鳃基因靶点表现出低氧诱导的表达变化。分析剑鱼的数据以确定那些表现出显著(p
Gene expression profiling using DNA microarray technology is a useful tool for assessing gene transcript level responses after an organism is exposed to environmental stress. Herein, we detail results from studies using an 8 k medaka (Oryzias latipes) microarray to assess modulated gene expression patterns upon hypoxia exposure of the live-bearing aquaria fish, Xiphophorus maculatus. To assess the reproducibility and reliability of using the medaka array in cross-genus hybridization, a two-factor ANOVA analysis of gene expression was employed. The data show the tissue source of the RNA used for array hybridization contributed more to the observed response of modulated gene targets than did the species source of the RNA. In addition, hierarchical clustering via heat map analyses of groupings of tissues and species (Xiphophorus and medaka) suggests that hypoxia induced similar responses in the same tissues from these two diverse aquatic model organisms. Our Xiphophorus results indicate 206 brain, 37 liver, and 925 gill gene targets exhibit hypoxia induced expression changes. Analysis of the Xiphophorus data to determine those features exhibiting a significant (p