Interaction of the European genotype porcine reproductive and respiratory syndrome virus (PRRSV) with sialoadhesin (CD169/Siglec-1) inhibits alveolar macrophage phagocytosis.
Interaction of the European genotype porcine reproductive and respiratory syndrome virus (PRRSV) with sialoadhesin (CD169/Siglec-1) inhibits alveolar macrophage phagocytosis.
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DOI：
10.1186/1297-9716-43-47
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发表时间：
2012-05-25
影响因子：
4.4
通讯作者：
Nauwynck HJ
中科院分区：
文献类型：
--
作者：
De Baere MI;Van Gorp H;Delputte PL;Nauwynck HJ
猪繁殖与呼吸综合征病毒（PRRSV）是一种动脉病毒，在体内对猪巨噬细胞亚群表现出有限的嗜性，肺泡巨噬细胞是主要的靶细胞。该病毒与所有年龄的猪的呼吸问题有关，通常在猪呼吸系统疾病复发区进行分离。由于病毒诱导的巨噬细胞在感染早期死亡，PRRSV阻碍了肺部对病原体的先天防御。此外，该病毒还可能直接影响巨噬细胞的抗菌功能。本研究探讨了欧洲基因型PRRSV与原代肺泡巨噬细胞（PAM）的相互作用是否会影响其吞噬能力。用I型PRRSV （LV）和III型PRRSV （Lena）分别接种巨噬细胞，结果显示病毒抑制PAM吞噬。使用灭活的PRRSV （LV）获得了类似的结果，表明病毒粒子与细胞的初始相互作用足以减少吞噬作用，并且不需要产生性感染。当巨噬细胞与两种病毒的进入介质唾液黏附素- (Sn)或CD163特异性抗体孵生时，只有Sn特异性抗体下调PAM的吞噬能力，这表明与Sn而不是CD163的相互作用介导了PRRSV对吞噬的抑制作用。综上所述，本研究表明，欧洲基因型PRRSV通过与其内化受体Sn的相互作用，在体外抑制PAM吞噬。如果类似的事件发生在体内，这种相互作用可能在PRDC的发展中很重要，正如在该领域经常看到的那样。
Porcine reproductive and respiratory syndrome virus (PRRSV) is an arterivirus that shows a restricted in vivo tropism for subsets of porcine macrophages, with alveolar macrophages being major target cells. The virus is associated with respiratory problems in pigs of all ages and is commonly isolated on farms with porcine respiratory disease complex (PRDC). Due to virus-induced macrophage death early in infection, PRRSV hampers the innate defence against pathogens in the lungs. In addition, the virus might also directly affect the antimicrobial functions of macrophages. This study examined whether interaction of European genotype PRRSV with primary alveolar macrophages (PAM) affects their phagocytic capacity. Inoculation of macrophages with both subtype I PRRSV (LV) and subtype III PRRSV (Lena) showed that the virus inhibits PAM phagocytosis. Similar results were obtained using inactivated PRRSV (LV), showing that initial interaction of the virion with the cell is sufficient to reduce phagocytosis, and that no productive infection is required. When macrophages were incubated with sialoadhesin- (Sn) or CD163-specific antibodies, two entry mediators of the virus, only Sn-specific antibodies downregulated the phagocytic capacity of PAM, indicating that interaction with Sn, but not CD163, mediates the inhibitory effect of PRRSV on phagocytosis. In conclusion, this study shows that European genotype PRRSV inhibits PAM phagocytosis in vitro, through the interaction with its internalization receptor Sn. If similar events occur in vivo, this interaction may be important in the development of PRDC, as often seen in the field.