Structural integrity of the Golgi is temperature sensitive in conditional-lethal mutants with no detectable GM130
Structural integrity of the Golgi is temperature sensitive in conditional-lethal mutants with no detectable GM130
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DOI：
10.1034/j.1600-0854.2003.00080.x
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发表时间：
2003-04-01
期刊：
影响因子：
4.5
通讯作者：
Krieger, M
中科院分区：
文献类型：
--
作者：
Vasile, E;Perez, T;Krieger, M
在温度敏感、条件致死的突变体IDIG中，在39.5摄氏度时，糖蛋白加工中断，分泌受阻。用免疫荧光和免疫电子显微镜观察IDIG细胞高尔基体的超微结构。在34℃时高尔基体结构正常，在39.5℃孵育12小时后，高尔基体解体为分散的囊泡。在34℃和39.5℃下，所有被检测的高尔基体标记物都在野生型水平上存在，GM130是检测不到的(
At 39.5degreesC in the temperature-sensitive, conditional-lethal mutant IdIG, glycoprotein processing is disrupted and secretion is blocked. The ultrastructure of the Golgi apparatus in IdIG cells was examined using immunofluorescence and immunoelectron microscopy. At 34 degreesC the structure of the Golgi apparatus was normal, whereas after incubation at 39.5 degreesC for 12 h it disassembled into dispersed vesicles. These reassembled into stacks when cells were returned to 34 degreesC for 6 h. At both 34 and 39.5 degreesC, all Golgi markers examined were present at wild-type levels except GM130, which was undetectable (