Articular cartilage repair in rabbits by using suspensions of allogenic chondrocytes in alginate
Articular cartilage repair in rabbits by using suspensions of allogenic chondrocytes in alginate
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DOI：
10.1016/s0142-9612(99)00241-0
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发表时间：
2000-04-01
期刊：
影响因子：
14
通讯作者：
Vittur, F
中科院分区：
文献类型：
--
作者：
Fragonas, E;Valente, M;Vittur, F
本研究旨在探讨藻酸盐凝胶中同种异体软骨细胞植入人工全层损伤兔关节软骨的可行性。通过将氯化钙溶液直接加入到缺损中，使藻酸盐中的软骨细胞悬浮液凝胶化，原位产生完全插入并粘附到软骨下骨和完整软骨壁的结构。在植入后1、2、4和6个月，通过NMR显微镜、同步辐射诱导的X射线发射以绘制糖胺聚糖的硫并通过组织化学控制组织修复。实际上，从软骨细胞植入4-6个月观察到缺损的完全修复，并恢复正常组织结构。单独植入藻酸钙的对照组仅形成纤维软骨。(C)2000爱思唯尔科技有限公司版权所有。
The feasibility of allogenic implants of chondrocytes in alginate gels was tested for the reconstruction in vivo of artificially full-thickness-damaged articular rabbit cartilage. The suspensions of chondrocytes in alginate were gelled by the addition of calcium chloride solution directly into the defects giving in situ a construct perfectly inserted and adherent to the subchondral bone and to the walls of intact cartilage. The tissue repair was controlled at 1, 2, 4 and 6 months after the implant by NMR microscopy, synchrotron radiation induced X-ray emission to map the sulfur of glycosaminoglycans and by histochemistry. Practically a complete repair of the defect was observed 4-6 months from the implant of the chondrocytes with the recovery of a normal tissue structure. Controls in which Ca-alginate alone was implanted developed only a fibrous cartilage. (C) 2000 Elsevier Science Ltd. All rights reserved.