Detection of Anisotropy in Cartilage Using 2H Double-Quantum-Filtered NMR-Spectroscopy
Detection of Anisotropy in Cartilage Using 2H Double-Quantum-Filtered NMR-Spectroscopy
复制标题
使用 2H 双量子过滤 NMR 波谱检测软骨的各向异性
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
G. Navon
中科院分区：
文献类型：
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作者：
Y. Sharf;U. Eliav;H. Shinar;G. Navon
I = 1自旋系统的双量子滤波（DQF）NMR光谱是检测宏观无序系统各向异性的诊断工具。对于氘，这种方法揭示了D2 O在牛鼻软骨中存在残余的四极相互作用。该组织在宏观上不有序，四极分裂未解决。拟合计算的光谱线型的实验结果是可能的，只有当分布的剩余四极相互作用，ω（q），是假定的。在DQF实验中，在不同的产生时间下得到的一系列DQF线形可以用一组三个参数来拟合：平均剩余四极相互作用ωq/2π = 110 Hz，标准差Δωq/2π = 73 Hz，横向弛豫速率63 s−1。单独的氘DQF测量的软骨，胶原蛋白和硫酸软骨素的成分表明，软骨的DQF光谱是由于结合到组织中的纤维胶原蛋白的D2 O的各向异性运动的结果。
Double-quantum-filtered (DQF) NMR spectroscopy of I = 1 spin systems is a diagnostic tool for the detection of anisotropy in macroscopically disordered systems. For deuterium, this method reveals the presence of a residual quadrupolar interaction for D2O in bovine nasal cartilage. This tissue is not macroscopically ordered and the quadrupolar splitting is not resolved. Fitting the calculated spectral lineshapes to the experimental results was possible only when a distribution of the residual quadrupolar interaction, omega(q), was assumed. The series of DQF lineshapes obtained for different creation times in the DQF experiment could be fitted using a single set of three parameters: the average residual quadrupolar interaction ωq/2π = 110 Hz, its standard deviation Δωq/2π = 73 Hz, and the transverse relaxation rate of 63 s−1. Separate deuterium DQF measurements for the constituents of the cartilage, collagen, and chondroitin sulfate indicated that the DQF spectra of cartilage are the result of anisotropic motion of D2O due to binding to the fibrous collagen in the tissue.