Osteoclast demise in the rat: Physiological versus degenerative cell death
Osteoclast demise in the rat: Physiological versus degenerative cell death
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DOI：
10.1113/expphysiol.1996.sp003929
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发表时间：
1996-03-01
影响因子：
2.7
通讯作者：
Dempster, DW
中科院分区：
文献类型：
--
作者：
Lutton, JD;Moonga, BS;Dempster, DW
时间推移视频显微镜被用来评估大鼠破骨细胞在暴露于各种促进细胞死亡的试剂后所经历的时间形态事件。直接观察发现，细胞死亡有两种形态上可区分的形式，分别类似于凋亡和坏死。在环孢素A、三苯氧胺、皮质酮和地塞米松等多种药物作用2-4小时后，细胞形态发生明显变化。细胞开始迅速萎缩，25min内出现圆形小球体。此时，细胞膜经历了剧烈的扭曲沸腾，伴随着小的膜芽的形成。细胞保持球形结构，膜似乎在几个小时内保持完好。这些观察结果与生理性细胞死亡或凋亡的过程相一致。在某些情况下，可见细胞凋亡后改变或继发性坏死，包括膜起泡和变性。相反，当细胞暴露在过氧化氢或叠氮化钠中时，细胞膜在1-4小时后明显恶化。这包括形成尖峰和/或气泡，并释放细胞内碎片，导致总体上的飞溅外观。这种类型的表现以细胞变性死亡或坏死为特征。对破骨细胞死亡模式的意义进行了讨论。
Time-lapse video microscopy was used to assess the temporal morphological events undergone by rat osteoclasts after exposure to a variety of agents that promote cell death. Direct observations revealed that there were two morphologically distinguishable forms of cell death, which resembled apoptosis and necrosis, respectively. Marked changes in morphology became apparent after 2-4 h exposure to a variety of agents, including cyclosporine A, tamoxifen, corticosterone and dexamethasone. The cells began to shrink rapidly and within 25 min appeared as small round spheres. At this time, the cell membranes underwent violent distortive boiling, or zeiosis, accompanied by the formation of small membrane buds. The cells maintained a spherical configuration and the membrane appeared to remain intact for several hours. These observations are consistent with the process of physiological cell death or apoptosis. In some cases, post-apoptotic changes or secondary necrosis could be seen, including membrane blebbing and degeneration. In contrast, when cells were exposed to hydrogen peroxide or sodium azide, there was a marked deterioration of the cell membrane after 1-4 h. This included the formation of spikes and/or blebbing with the release of intracellular debris, resulting in an overall spattered appearance. This type of appearance is characteristic of degenerative cell death or necrosis. The significance of the mode of osteoclast death is discussed.