In vivo and in vitro studies of genetic resistance to systemic salmonellosis in the chicken encoded by the SAL1 locus
In vivo and in vitro studies of genetic resistance to systemic salmonellosis in the chicken encoded by the SAL1 locus
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DOI：
10.1016/s1286-4579(02)01635-0
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发表时间：
2002-09-01
影响因子：
5.8
通讯作者：
Barrow, PA
中科院分区：
文献类型：
--
作者：
Wigley, P;Hulme, SD;Barrow, PA
许多鸡的近交系已被证明对由成禽肠道沙门氏菌（Salmonella enterica serovar Gallinarum）或沙门氏菌（S. enterica serovar enteritrium和S.雏鸡肠道血清型鼠伤寒。耐药品系仅显示出中度病理和低死亡率，而敏感品系在沙门氏菌感染后显示出广泛的病理变化和较高水平的死亡率。对沙门氏菌病的遗传耐药性是主导的，与性别、MHC或Slc 11 a1（以前称为Nramp 1）无关，这导致小鼠和其他物种产生耐药性。在鸡5号染色体上发现了一个新的编码沙门氏菌病抗性的基因座，命名为SALT。在体内抗性和敏感鸡品系之间发现的病理学差异的性质表明抗性在单核吞噬细胞系统的水平上表达。来自成年抗性品系鸟的巨噬细胞在24小时内从感染的巨噬细胞中清除了鸡血清沙门氏菌，而沙门氏菌在来自敏感品系鸟的巨噬细胞中持续存在至少48小时。沙门氏菌的清除巨噬细胞伴随着一个强大的和可重复的呼吸爆发反应，在耐药线，但很少或没有响应敏感线。来自远交系鸡的巨噬细胞表现出可变的反应。在来自抗性或敏感品系的细胞中，巨噬细胞一氧化氮的产生没有差异。这些差异表明，增加的巨噬细胞抗菌活性与抗性相关，巨噬细胞活性在鸡对系统性沙门氏菌病的遗传抗性中起重要作用。(C)2002年，Elsevier SAS科学与医学版。All rights reserved.
A number of inbred lines of chickens have been shown to be resistant or susceptible to systemic salmonellosis caused by Salmonella enterica serovar Gallinarum in adult birds, or by S. enterica serovar Enteritidis and S. enterica serovar Typhimurium in young chicks. Resistant lines show only moderate pathology and low mortality rates, whereas susceptible lines display extensive pathological changes and higher levels of mortality following Salmonella infection. Genetic resistance to salmonellosis is dominant and not linked to sex, MHC or Slc11a1 (formerly known as Nramp1), which leads to resistance in mice and other species. A novel locus encoding resistance to salmonellosis has been identified on chicken chromosome 5, and designated SALT. The nature of the differences in pathology found between resistant and susceptible chicken lines in vivo indicates that resistance is expressed at the level of the mononuclear phagocyte system. Macrophages from adult resistant line birds cleared Salmonella serovar Gallinarum from infected macrophages within 24 h, whereas Salmonella bacteria persisted within macrophages from susceptible line birds for at least 48 h. Clearance of Salmonella by macrophages was accompanied by a strong and reproducible respiratory burst response in resistant lines, but little or no response in susceptible lines. Macrophages from an outbred chicken line showed variable responses. No differences were seen in macrophage nitric oxide production in cells from resistant or susceptible lines. These differences suggest that increased macrophage antimicrobial activity correlates with resistance and that macrophage activity plays an important role in genetic resistance to systemic salmonellosis in the chicken. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.