Prolonged support of working rabbit hearts using Fluosol-43 or erythrocyte media.
Prolonged support of working rabbit hearts using Fluosol-43 or erythrocyte media.
复制标题
使用 Fluosol-43 或红细胞培养基延长兔心脏的工作时间。
DOI：
10.1152/ajpheart.1987.252.2.h349
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Boyle3rd,WA
中科院分区：
文献类型：
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作者：
Segel,LD;Ensunsa,JL;Boyle3rd,WA
我们比较了全氟化学乳液Fluosol-43和红细胞为基础的解决方案作为离体工作兔心脏功能与生理工作量的支持媒体。两组心脏（n = 5/组）在灌注的前6小时表现出稳定的功能（左心室收缩压峰值、左心室压力上升和舒张的峰值速率、主动脉流量、主动脉流量峰值、每搏作功和峰值功率）。在最初的6小时内，两组的冠状动脉流量、冠状静脉O2含量和O2供需比下降相似。两组心脏都优先利用丙酮酸而不是葡萄糖。Fluosol-43灌注心脏的心率、左心室收缩压峰值、左心室压力升高峰值速率、主动脉血流量、冠状动脉血流量和心肌耗氧量均高于红细胞灌注心脏。Fluosol-43心脏产生更多的乳酸和释放更多的肌酸磷酸激酶比红细胞灌注的心脏，但速率低，并在整个灌注恒定，表明心脏不是渐进性缺血。在第一个6小时后，Fluosol-43心脏的功能下降，导致与红细胞灌注的心脏相比，它们更早地衰竭。数据表明，Fluosol-43有足够的O2-携带能力，以支持稳定的功能，兔心脏在生理工作负荷6小时，功能和离体寿命的两组心脏的差异表明，一个组件或污染物的Fluosol-43改变肌膜功能和/或膜完整性所需的组件是缺乏的Fluosol-43灌注液。
We compared the perfluorochemical emulsion Fluosol-43 and an erythrocyte-based solution as support media for ex vivo working rabbit hearts functioning with a physiological workload. Both groups of hearts (n = 5/group) exhibited stable function (left ventricular peak systolic pressure, peak rates of left ventricular pressure rise and relaxation, aortic flow, peak aortic flow rate, stroke work, and peak power) for the first 6 h of perfusion. Coronary flow, coronary venous O2 content, and O2 supply-to-demand ratio declined similarly in both groups during the first 6 h. Both groups of hearts preferentially utilized pyruvate to glucose. The Fluosol-43-perfused hearts had higher heart rate, left ventricular peak systolic pressure, peak rate of left ventricular pressure rise, aortic flow, coronary flow, and myocardial O2 consumption compared with the erythrocyte-perfused hearts. The Fluosol-43 hearts produced more lactate and released more creatine phosphokinase than did the erythrocyte-perfused hearts, but the rates were low and constant throughout perfusion, indicating that the hearts were not progressively ischemic. After the first 6 h, function of the Fluosol-43 hearts declined, resulting in their earlier failure compared with the erythrocyte-perfused hearts. The data indicate that Fluosol-43 had sufficient O2- carrying capacity to support stable function of a rabbit heart at a physiological workload for 6 h, and differences in function and ex vivo longevity of the two groups of hearts suggested that a component or contaminant of Fluosol-43 altered sarcolemmal function and/or that a component needed for membrane integrity was lacking in the Fluosol-43 perfusate.