POSTNATAL-DEVELOPMENT OF MULTIPLE OPIOID RECEPTORS IN RAT-BRAIN
POSTNATAL-DEVELOPMENT OF MULTIPLE OPIOID RECEPTORS IN RAT-BRAIN
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DOI：
10.1016/0165-3806(87)90226-4
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发表时间：
1987-12-15
期刊：
影响因子：
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通讯作者：
LESLIE, FM
中科院分区：
文献类型：
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作者：
KORNBLUM, HI;HURLBUT, DE;LESLIE, FM
最近的研究表明，阿片受体可能在个体发育的早期阶段发挥作用，并可能调节特定的发育功能。然而，目前尚不清楚可能涉及哪种特定的阿片受体亚型。在本研究中，我们使用选择性放射性配体结合条件与定量放射自显影相结合来检查发育中的大鼠大脑中μ-、κ-和δ-阿片受体的个体发育。膜结合数据表明，在新生大鼠和成年大鼠中，μ-、κ-和δ-位点对放射性标记药物的亲和力相似。 mu.-和κ-受体在新生儿早期以显着的密度存在，而δ-受体出现得晚得多。放射自显影数据表明，mu.-和kappa.-受体出现在几个大脑区域的发育早期，包括新纹状体、嗅结节和中脑吻端，随后出现在其他区域，例如丘脑和下丘脑。尽管κ结合位点的密度在整个发育过程中保持相对恒定，但在几个大脑区域中存在μ受体的短暂出现和/或重新分布。出生时，δ-受体以低密度存在于基底前脑中。在出生后第三周期间，在所有检查的脑区域中δ-受体结合水平显着增加。 mu-和kappa-受体在大脑个体发育过程中的早期出现表明这些受体至少部分地介导外源性和内源性阿片类药物的发育作用。
Recent studies have demonstrated that opioid receptors may be functional at early stages of ontogeny, and may modulate specific developmental functions. It is presently unknown, however, which particular opioid receptor subtype(s) may be involved. In the present study, we have used selective radioligand binding conditions in combination with quantitative autoradiography to examine the ontogeny of .mu.-, .kappa.- and .delta.-opioid receptors in the developing rat brain. Membrane binding data indicate that the affinities of .mu.-, .kappa.- and .delta.-sites for radiolabeled drugs are similar in neonatal and adult rats. .mu.- and .kappa.-receptors are present in significant densities during early neonatal periods, while .delta.-receptors appear much later. Autoradiographic data indicate that .mu.- and .kappa.-receptors appear early in the development of several brain regions, including the neostriatum, olfactory tubercle and rostral midbrain, and later in other regions such as the thalamus and hypothalamus. Whereas the densities of .kappa.-binding sites remain relatively constant throughout development, there is a transient appearance and/or redistribution of .mu.-receptors in several brain areas. .delta.-Receptors are present in low densities in the basal forebrain at birth. The level of .delta.-receptor binding increases markedly during the third postnatal week in all brain areas examined. The early appearance of .mu.- and .kappa.-receptors during the ontogeny of the brain suggests that these receptors, at least in part, mediate the developmental actions of exogenous and endogenous opioids.