Seroprevalence of SARS-CoV-2 Antibodies in Children and Adults in St. Louis, Missouri, USA.
Seroprevalence of SARS-CoV-2 Antibodies in Children and Adults in St. Louis, Missouri, USA.
复制标题
美国密苏里州圣路易斯儿童和成人中SARS-COV-2抗体的血清阳性。
DOI：
10.1128/msphere.01207-20
复制
发表时间：
2021-02-03
期刊：
影响因子：
4.8
通讯作者：
Wang D
中科院分区：
文献类型：
--
作者：
Smith BK;Janowski AB;Danis JE;Harvey IB;Zhao H;Dai YN;Farnsworth CW;Gronowski AM;Roper S;Fremont DH;Wang D
这项研究确定了2020年4月底至5月初圣路易斯大都会区儿童和成人样本中对SARS-CoV-2有抗体的百分比。报告的2019年冠状病毒病（COVID-19）病例数可能低估了真实的患病率，因为轻度或无症状病例往往未经检测。在这里，我们使用一个血清调查，以估计IgG抗体的血清阳性率严重急性呼吸综合征冠状病毒2（SARS-CoV-2）在圣路易斯，密苏里州，大都会区在一个独立的方式。从2020年4月14日至2020年5月12日期间前往巴恩斯犹太医院或圣路易斯儿童医院就诊的患者中采集了503份成人和555份儿童血清/血浆样本。我们开发了使用加标物和核蛋白的内部酶联免疫吸附测定（ELISA）方案，并使用该测定来估计基于我们样本的血清阳性率。儿童样本中总体IgG血清阳性率估计为1.71%（95%可信区间[CI]，0.04%至3.38%），成人样本中为3.11%（95% CI，0.92%至5.32%）。5岁以下儿童的血清阳性率明显低于成人，但成人与5岁或5岁以上儿童的血清阳性率相似。在对176个4岁以下儿童的样本进行检测后，没有一个样本呈阳性。这项研究确定了2020年4月下旬至5月上旬圣路易斯大都会区儿童和成人样本中有SARS-CoV-2抗体的百分比。大约1.7%至3.1%的受试者有抗体，表明他们以前曾感染过SARS-CoV-2。这些结果表明，感染的程度比当时确诊病例的数量高出约10倍。此外，它表明，到5岁时，儿童的感染程度与成人相似。
This study determined the percentages of both children and adult samples from the greater St. Louis metropolitan area who had antibodies to SARS-CoV-2 in late April to early May 2020. Reported coronavirus disease 2019 (COVID-19) case counts likely underestimate the true prevalence because mild or asymptomatic cases often go untested. Here, we use a sero-survey to estimate the seroprevalence of IgG antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the St. Louis, MO, metropolitan area in a symptom-independent manner. Five hundred three adult and 555 pediatric serum/plasma samples were collected from patients presenting to Barnes-Jewish Hospital or St. Louis Children’s Hospital between 14 April 2020 and 12 May 2020. We developed protocols for in-house enzyme-linked immunosorbent assays (ELISAs) using spike and nucleoprotein and used the assays to estimate a seroprevalence rate based on our samples. Overall IgG seropositivity was estimated to be 1.71% (95% credible interval [CI], 0.04% to 3.38%) in pediatric samples and 3.11% (95% CI, 0.92% to 5.32%) in adult samples. Seropositivity was significantly lower in children under 5 years of age than in adults, but rates between adults and children aged 5 or older were similar. Of the 176 samples tested from children under 4 years of age, none were positive. IMPORTANCE This study determined the percentages of both children and adult samples from the greater St. Louis metropolitan area who had antibodies to SARS-CoV-2 in late April to early May 2020. Approximately 1.7 to 3.1% of the tested individuals had antibodies, indicating that they had previously been infected by SARS-CoV-2. These results demonstrate that the extent of infection was about 10 times greater than the number of confirmed cases at that time. Furthermore, it demonstrated that by 5 years of age, children were infected to an extent similar to that of adults.