Early intestinal development and mucin transcription in the young poult with probiotic and mannan oligosaccharide prebiotic supplementation
Early intestinal development and mucin transcription in the young poult with probiotic and mannan oligosaccharide prebiotic supplementation
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DOI：
10.3382/ps/pew019
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发表时间：
2016-05-01
期刊：
影响因子：
4.4
通讯作者：
Lilburn, M. S.
中科院分区：
文献类型：
--
作者：
Hutsko, S. L.;Meizlisch, K.;Lilburn, M. S.
减少饮食抗生素使用的替代和辅助方法正在成为流行的研究领域。补充益生菌(共生微生物)和益生菌(难消化的复杂碳水化合物)是促进有益细菌在肠道定植的两种饮食方法，以与潜在的病原体竞争，从而创造健康的粘膜环境。肠粘膜由粘蛋白糖蛋白组成，在防止病原菌附着方面起着关键作用。在孵化阶段，新生火鸡的肠道相对无菌，容易受到共生微生物和病原微生物的细菌定植。在本研究中，我们测定了小肠内杯状细胞产生的主要粘蛋白MUC2的转录，并测量了出生后即刻至第11天的肠道形态。雏鸡饲喂常规启动子日粮、添加两种商业益生菌(A、B)之一的启动子日粮或商业甘露低聚糖。MUC2转录从d0增加到d4(P<；0.05)，但添加益生素或益生素没有影响。添加益生菌B和甘露寡糖均能增加绒毛高度和绒毛面积(P<；0.05)，且对隐窝深度有显著的交互作用(P=0.007)。这些结果表明，益生菌和益生菌的补充可以积极改变肠道微环境。
Alternative and adjunctive approaches to decreasing the use of dietary antibiotics are becoming popular areas of study. Supplemental probiotics (commensal microbes) and prebiotics (indigestible complex carbohydrates) are 2 dietary approaches to facilitating the intestinal colonization of beneficial bacteria to compete with potential pathogens, thus creating a healthy mucosal environment. The intestinal mucosa is composed of mucin glycoproteins, which play a key role in preventing the attachment of pathogenic bacteria. At hatch, the neonatal turkey intestine is relatively aseptic and vulnderable to bacterial colonization by both commensal and pathogenic microbes. In the current study, we determined the transcription of MUC2, the primary mucin protein produced by goblet cells within the small intestine, and we also measured intestinal morphology immediately posthatch through d 11. Poults were fed a conventional starter diet, the starter diet supplemented with one of 2 commercial probiotics (A, B), or a commercial mannan oligosaccharide. MUC2 transcription increased from d zero to d 4 posthatch (P < 0.05), but there was no effect of probiotic or prebiotic supplementation. Villus height and villus area both increased with Probiotic B and mannan oligosaccharide supplementation (P < 0.05) and there was a significant d X treatment interaction effect for crypt depth (P = 0.007). These results suggest that probiotic and prebiotic supplementation can positively alter the intestinal microenvironment.