Therapy of Mycobacterium avium complex infections in beige mice with streptomycin encapsulated in sterically stabilized liposomes.
Therapy of Mycobacterium avium complex infections in beige mice with streptomycin encapsulated in sterically stabilized liposomes.
复制标题
用封装在空间稳定脂质体中的链霉素治疗米色小鼠的鸟分枝杆菌复合体感染。
DOI：
10.1128/aac.39.3.725
复制
发表时间：
1995
影响因子：
4.9
通讯作者：
Düzgüneş,N
中科院分区：
文献类型：
--
作者：
Gangadharam,PR;Ashtekar,DR;Flasher,DL;Düzgüneş,N
鸟分枝杆菌复合体（MAC）可引起AIDS患者严重的机会性感染。先前对MAC感染的米色小鼠的研究表明，每周给予脂质体包封的链霉素可显著降低肝脏和脾脏中的CFU。我们研究了是否链霉素封装在最近开发的空间稳定的脂质体，延长循环时间将在这种动物模型的治疗效果。两种延长循环的脂质体类型（聚乙二醇-二硬脂酰磷脂酰乙醇胺[PEG-DSPE]-二硬脂酰磷脂酰胆碱[DSPC]-胆固醇[chol]或磷脂酰肌醇[PI]-DSPC-chol）和常规脂质体（磷脂酰甘油[PG]-磷脂酰胆碱[PC]-chol）当治疗后的感染水平与对照组相比时，治疗前的感染水平。包封链霉素的PI-DSPC-chol和PG-PC-chol脂质体在肝脏中具有杀菌作用。虽然PG-PC-chol或PEG-DSPE-DSPE-chol脂质体包封链霉素在肺中不具有杀菌作用，但与未处理对照组的MAC感染水平相比，它们使MAC感染水平降低了3个数量级以上。
Mycobacterium avium complex (MAC) causes serious opportunistic infections in AIDS patients. Previous studies with MAC-infected beige mice have indicated that weekly administration of liposome-encapsulated streptomycin can reduce significantly the CFU in the liver and spleen. We examined whether streptomycin encapsulated in recently developed sterically stabilized liposomes with prolonged circulation times would have a therapeutic effect in this animal model. Two liposome types with prolonged circulation (polyethyleneglycol-distearoylphosphatidylethanolamine [PEG-DSPE]-distearoylphosphatidylcholine [DSPC]-cholesterol [chol] or phosphatidylinositol [PI]-DSPC-chol) and conventional liposomes (phosphatidylglycerol [PG]-phosphatidylcholine [PC]-chol) encapsulating streptomycin and administered twice weekly were bactericidal to MAC strain 101 in the spleen when the level of infection after treatment was compared with the level of infection before treatment. PI-DSPC-chol and PG-PC-chol liposomes encapsulating streptomycin were bactericidal in the liver. Although PG-PC-chol or PEG-DSPE-DSPE-chol liposomes encapsulating streptomycin were not bactericidal in the lungs, they reduced the level of MAC infection by more than 3 orders of magnitude compared with the level of MAC infection in untreated controls.