WATER PERMEABILITY AND ITS ACTIVATION-ENERGY OF FERTILIZED AND UNFERTILIZED MOUSE OVA
WATER PERMEABILITY AND ITS ACTIVATION-ENERGY OF FERTILIZED AND UNFERTILIZED MOUSE OVA
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DOI：
10.1007/bf01868823
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发表时间：
1980-01-01
影响因子：
2.4
通讯作者：
LEIBO, SP
中科院分区：
文献类型：
--
作者：
LEIBO, SP
一个显微镜的方法被用来确定在不同的恒温下从受精和未受精的小鼠卵的水损失的动力学。将卵子转移到高渗盐水溶液中，拍照，并根据转移后横截面积与时间的函数关系计算其体积。描述所观察到的H2O损失的曲线进行了比较，使用编程版本的经典H2O渗透率方程计算。通过在计算中改变其值，直到大多数观察到的细胞体积落在或非常接近计算的体积对时间的曲线，来确定水力传导率。以这种方式，受精和未受精的卵在20 ℃下分别具有0.43和0.44 μ m3/μ m2-min-atm的水力传导率。C和相应的活化能为13.0和14.5千卡/摩尔的H2O渗透。
A photomicroscopic method was used to determine the kinetics of H2O loss at various constant temperatures from fertilized and unfertilized mouse ova. Ova were transferred into hypertonic saline solutions, photographed and their volumes were calculated from their cross-sectional areas as a function of time after transfer. Curves describing the observed H2O loss were compared to those calculated using a programmed version of the classical H2O permeability equation. The hydraulic conductivity was determined by changing its value in the calculation until the majority of the observed cell volumes fell on or very close to the calculated curve of volume vs. time. In this way, fertilized and unfertilized ova had respective hydraulic conductivities of 0.43 and 0.44 .mu.m3/.mu.m2-min-atm at 20.degree. C and respective activation energies for H2O permeability of 13.0 and 14.5 kcal/mol.