Dynamic mechanical consequences of deep inflation in mice depend on type and degree of lung injury
Dynamic mechanical consequences of deep inflation in mice depend on type and degree of lung injury
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DOI：
10.1152/japplphysiol.00270.2003
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发表时间：
2004-01-01
影响因子：
3.3
通讯作者：
Bates, JHT
中科院分区：
文献类型：
--
作者：
Allen, G;Bates, JHT
在先前的研究中（艾伦G，Lundblad LK，Parsons P和Bates JH.第93章：我的天1709 - 1715，2002），我们的实验室在小鼠中使用深度充气（DI）来显示闭合肺单位的募集在肺损伤中可以是非常短暂的现象。本研究的目的是探讨这种短暂的肺复张如何取决于急性肺损伤的性质和程度。给小鼠施用50 μ l盐水（n = 8）、0.01M（n = 9）或0.025M（n = 8）盐酸，或50 μ g（n = 10）或150 μ g（n = 6）LPS，并在24 - 48小时后进行机械通气。在不同水平的呼气末正压下，输送两次DI，并使用强制振荡在7分钟内周期性地获得肺硬度（H）的测量值。在LPS暴露后，DI后H的压力-容积曲线滞后和恢复与盐水暴露对照没有什么不同，尽管支气管肺泡灌洗液中的中性粒细胞多了500倍。酸暴露小鼠的压力-容积滞后和H的恢复增加（P < 0.001），并与支气管肺泡灌洗液蛋白含量相关（R = 0.81）。呼气末正压降低了所有组的H恢复（P < 0.01），但仅降低了酸损伤组的压力-容积滞后（P < 0.001）。我们的结论是，DI在急性肺损伤中的作用取决于肺损伤的程度，但仅限于这种损伤反映了气液界面的破坏。
In a previous study ( Allen G, Lundblad LK, Parsons P, and Bates JH. J Appl Physiol 93: 1709 - 1715, 2002), our laboratory used deep inflations ( DI) in mice to show that recruitment of closed lung units can be a very transient phenomenon in lung injury. The purpose of this study was to investigate how this transience of lung recruitment depends on the nature and degree of acute lung injury. Mice were administered 50 mu l of either saline ( n = 8), 0.01 M ( n = 9) or 0.025 M ( n = 8) hydrochloric acid, or 50 mug ( n = 10) or 150 mug ( n = 6) of LPS and were mechanically ventilated 24 - 48 h later. At various levels of positive end- expiratory pressure, two DIs were delivered, and forced oscillations were used to obtain a measure of lung stiffness ( H) periodically over 7 min. After LPS exposure, pressure- volume curve hysteresis and recovery in H after DI were no different from saline- exposed controls despite 500 times more neutrophils in bronchoalveolar lavage fluid. Pressure- volume hysteresis and recovery in H were increased in acid- exposed mice ( P < 0.001) and were correlated with bronchoalveolar lavage fluid protein content ( R = 0.81). Positive end- expiratory pressure reduced recovery in H in all groups ( P < 0.01) but reduced pressure- volume hysteresis in the acid- injured groups only ( P < 0.001). We conclude that the effects of DIs in acute lung injury depend on the degree of lung injury but only to the extent that this injury reflects a disruption of the air- liquid interface.