METABOLISM OF R-BETA-HYDROXYPENTANOATE AND OF BETA-KETOPENTANOATE IN CONSCIOUS DOGS
METABOLISM OF R-BETA-HYDROXYPENTANOATE AND OF BETA-KETOPENTANOATE IN CONSCIOUS DOGS
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DOI：
10.1152/ajpendo.1995.268.3.e446
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发表时间：
1995-03-01
影响因子：
5.1
通讯作者：
BRUNENGRABER, H
中科院分区：
文献类型：
--
作者：
LECLERC, J;ROSIERS, CD;BRUNENGRABER, H
R-β-羟基戊酸和β-酮戊酸是生理酮体R-β-羟基丁酸和乙酰乙酸的同系物。其来源于肝脏中R，S-1，3-戊二醇（一种潜在的营养物质）的R-部分的氧化。本报告记录了清醒犬中R-β-羟基戊酸和β-酮戊酸的代谢。无论是通过推注还是恒定输注给药，这两种底物都会相互转化并迅速代谢。当β-酮戊酸以相当于狗的热量需求的75%的速率输注时，两种底物的稳态总血浆浓度仅为1.3 mM。由于底物是丙酰辅酶A的前体，我们测定了丙酸血症标志物的尿浓度。与丙酸血症患者中观察到的情况相比，其蓄积量较小。我们的结论是，至少在短期实验中，R-β-羟基戊酸和β-酮戊酸在狗中代谢良好，对大量供应的丙酰辅酶A没有明显的不耐受性。
R-beta-Hydroxypentanoate and beta-ketopentanoate are homologues of physiological ketone bodies R-beta-hydroxybutyrate and acetoacetate. They derive from the oxidation in liver of the R-moiety of R,S-1,3-pentanediol, a potential nutrient. This report documents the metabolism of R-beta-hydroxypentanoate and beta-ketopentanoate in conscious dogs. Whether administered by bolus or constant infusion, the two substrates are interconverted and rapidly metabolized. When beta-ketopentanoate was infused at a rate corresponding to 75% of the dog's caloric requirement, the steady-state total plasma concentration of the two substrates was only 1.3 mM. Because the substrates are precursors of propionyl-CoA, we assayed the urinary concentrations of markers of propionic acidemia. Their accumulation was minor compared with what is observed in patients suffering from propionic acidemia. We conclude that, at least during short-term experiments, R-beta-hydroxypentanoate and beta-ketopentanoate are well metabolized in the dog without apparent intolerance to a large supply of propionyl-CoA.