Microbiome Variation Across Two Hemlock Species With Hemlock Woolly Adelgid Infestation
Microbiome Variation Across Two Hemlock Species With Hemlock Woolly Adelgid Infestation
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DOI：
10.3389/fmicb.2020.01528
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发表时间：
2020-07-07
影响因子：
5.2
通讯作者：
Cregger, Melissa A.
中科院分区：
文献类型：
--
作者：
Dove, Nicholas C.;Rogers, Timothy J.;Cregger, Melissa A.
铁杉绵蚜（Adelges tsugae， HWA）是一种入侵昆虫，对北美东部的本地铁杉种群造成了毁灭性的破坏，迄今为止，管理结果收效甚微。虽然许多植物微生物组影响甚至支持植物对昆虫食草的免疫反应，但对铁杉微生物组及其与病原体或草食动物（如HWA）的相互作用知之甚少。利用16S rRNA和ITS基因扩增子测序技术，对两种铁杉（Tsuga canadensisandT）的针、枝、根和根际微生物群进行了分析。三叶虫，显示出低和高水平的HWA种群。研究发现，古菌/细菌群落和真菌针叶群落以及古菌/细菌枝和根群落的组成在两种铁杉种群中均存在差异。虽然寄主物种和植物相关栖息地比HWA种群水平解释了微生物组变异的更大比例，但与HWA种群较低的树木相比，HWA种群高的树木在四种植物相关栖息地（如针叶、树枝、根、根际）中与100种可能的真菌病原体序列变异相关。这项工作有助于越来越多的文献将植物病原体和害虫与相关植物微生物组和宿主健康的变化联系起来。此外，这项工作表明需要进一步研究植物微生物组在多种植物组织中的作用，以了解它们对宿主健康的影响。
The hemlock woolly adelgid (Adelges tsugae, HWA), an invasive insect, is devastating native hemlock populations in eastern North America, and management outcomes have so far had limited success. While many plant microbiomes influence and even support plant immune responses to insect herbivory, relatively little is known about the hemlock microbiome and its interactions with pathogens or herbivores such as HWA. Using 16S rRNA and ITS gene amplicon sequencing, we characterized the needle, branch, root, and rhizosphere microbiome of two hemlock species,Tsuga canadensisandT. sieboldii, that displayed low and high levels of HWA populations. We found that both archaeal/bacterial and fungal needle communities, as well as the archaeal/bacterial branch and root communities, varied in composition in both hemlock species relative to HWA population levels. While host species and plant-associated habitats explained a greater proportion of the variance in the microbiome than did HWA population level, high HWA populations were associated with enrichment of 100 likely fungal pathogen sequence variants across the four plant-associated habitats (e.g., needle, branch, root, rhizosphere) compared to trees with lower HWA populations. This work contributes to a growing body of literature linking plant pathogens and pests with the changes in the associated plant microbiome and host health. Furthermore, this work demonstrates the need to further investigate plant microbiome effects across multiple plant tissues to understand their influences on host health.