Rapid synthesis of stable and functional conjugates of DNA/gold nanoparticles mediated by Tween 80.
Rapid synthesis of stable and functional conjugates of DNA/gold nanoparticles mediated by Tween 80.
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DOI：
10.1021/la203632c
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发表时间：
2011-10
期刊：
影响因子：
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通讯作者：
Shengmin Xu;Hang Yuan;A. Xu;Jun Wang;Lijun Wu
中科院分区：
文献类型：
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作者：
Shengmin Xu;Hang Yuan;A. Xu;Jun Wang;Lijun Wu
与DNA结合的金纳米颗粒代表了在生物传感器、医学诊断和生物分析中广泛应用的有吸引力的替代平台。然而，目前将DNA与金纳米颗粒缀合的方法是耗时的。在这项研究中，我们报告了一种新的方法，快速缀合DNA的金纳米粒子（AuNPs），形成功能性DNA/AuNPs在2-3小时使用吐温80作为保护剂。利用基于荧光的技术，我们确定AuNP表面上的DNA密度达到约1060股/颗粒，这与当前方法中的装载密度相当。此外，通过我们的方法合成的DNA/AuNPs表现出优异的稳定性作为温度，pH值和冻融循环的函数，并且DNA/AuNPs缀合物的功能性也得到了验证。本文的工作对开发快速、可重复合成稳定的DNA功能化金纳米粒子具有重要意义。
Gold nanoparticles conjugated with DNA represent an attractive and alternative platform for broad applications in biosensors, medical diagnostic, and biological analysis. However, current methods to conjugate DNA to gold nanoparticles are time-consuming. In this study, we report a novel approach to rapidly conjugate DNA to gold nanoparticles (AuNPs) to form functional DNA/AuNPs in 2-3 h using Tween 80 as protective agent. With a fluorescence-based technique, we determine that the DNA density on the surface of AuNPs achieves about ∼60 strands per particles, which is comparable to the loading density in the current methods. Moreover, the DNA/AuNPs synthesized by our approach exhibit an excellent stability as a function of temperature, pH, and freeze-thaw cycle, and the functionality of DNA/AuNPs conjugates is also verified. The work presented here has important implications to develop the fast and reproducible synthesis of stable DNA-functionalized gold nanoparticles.