OSTEOBLASTIC CELLS MEDIATE OSTEOCLASTIC RESPONSIVENESS TO PARATHYROID-HORMONE
OSTEOBLASTIC CELLS MEDIATE OSTEOCLASTIC RESPONSIVENESS TO PARATHYROID-HORMONE
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DOI：
10.1210/endo-118-2-824
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发表时间：
1986-02-01
期刊：
影响因子：
4.8
通讯作者：
CHAMBERS, TJ
中科院分区：
文献类型：
--
作者：
MCSHEEHY, PMJ;CHAMBERS, TJ
间接证据表明，成骨细胞系可能介导PTH诱导的骨吸收增强。为了验证这一观点，我们将新生大鼠长骨中的破骨细胞分解，并在人股骨皮质骨切片上培养。通过计算机辅助的形态测量和立体摄影定量的骨细胞挖掘，确定在培养后的扫描电子显微镜。我们比较了甲状旁腺素对单独培养的破骨细胞骨吸收的影响，以及甲状旁腺素对与成骨细胞共培养的破骨细胞骨吸收的影响。甲状旁腺素对单独的破骨细胞的骨吸收没有影响，但在任何三个含成骨细胞的细胞群的存在下，或在克隆的骨肉瘤细胞的存在下，甲状旁腺素引起骨吸收增加2至4倍。在10-4 IU/ml PTH下观察到显著刺激。在没有破骨细胞的情况下，成骨细胞群均未引起形态学上可检测到的骨吸收。这些结果表明，PTH主要作用于成骨细胞，成骨细胞由激素的存在诱导，以刺激骨细胞的骨吸收。
Indirect evidence suggests that cells of the osteoblastic lineage may mediate augmented osteoclastic bone resorption induced by PTH. To test this suggestion, osteoclasts were disaggregated from neonatal rat long bones and incubated on slices of human femoral cortical bone. Resorption was measured by computer-assisted morphometric and stereophotogrammetric quantificaton of osteoclastic excavations, identified in the scanning electron microscope after culture. We compared the effect of PTH on bone resorption by osteoclasts incubated alone with the effect of the hormone on resorption by osteoclasts cocultured with osteoblastic cells. PTH had no effect on bone resorption by osteoclasts alone, but in the presence of any of three osteoblast-containing cell populations, or in the presence of cloned, hormone-responsive osteosarcoma cells, PTH caused a 2- to 4-fold increase in osteoclastic resorption. Significant stimulation was observed at 10-4 IU/ml PTH. None of the osteoblastic cell populations caused morphologically detectable bone resorption in the absence of osteoclasts. These results indicate that PTH acts primarily on osteoblasts, which are induced by the presence of the hormone to stimulate osteoclastic bone resorption.